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CB / CB-UL, CBF / CBF-UL (E7) 07/2021 Page 6/197
19.1.3
Measuring CO
2
directly with an electronic infrared measuring device ................................. 146
19.2 Temperature reference measurement ............................................................................................ 146
20. AVOIDING MICROBIAL CONTAMINATION...................................................... 147
20.1 Cells and media .............................................................................................................................. 147
20.2 Laboratory conditions / equipment around the incubator ................................................................ 147
20.3 Working and behavior in the lab ..................................................................................................... 147
20.4 Chamber design and equipment of the CO
2
incubator ................................................................... 148
20.5 Handling the CO
2
incubator ............................................................................................................ 149
21. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 150
21.1 Cleaning .......................................................................................................................................... 150
21.2 Decontamination / chemical disinfection of the chamber ................................................................ 152
21.2.1 Disinfection of the CO
2
sensor .............................................................................................. 153
21.2.2 CBF / CBF-UL: Disinfection of components of the fresh water supply system .................... 153
21.3 Hot-air sterilization .......................................................................................................................... 154
21.3.1 Overview ............................................................................................................................... 154
21.3.2 Preparation for a hot-air sterilization ..................................................................................... 155
21.3.3 Starting the sterilization cycle and running the hot-air sterilization ....................................... 155
21.3.4 Prematurely terminating the sterilization cycle ..................................................................... 157
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective
sterilization .................................................................................................................... 157
21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the
cooling-down phase: successful sterilization ................................................................ 158
21.3.5 Completing the entire sterilization cycle ............................................................................... 159
22. MAINTENANCE AND SERVICE, TROUBLESHOOTING, REPAIR, TESTING . 160
22.1 General information, personnel qualification................................................................................... 160
22.2 CBF / CBF-UL: Replacing components of the humidifying / dehumidifying system ....................... 161
22.2.1 Replacing the silicone tube of the fresh water supply system .............................................. 161
22.2.2 Replacing the pump head of the fresh water supply system ................................................ 161
22.2.3 Replacing the gas fine filter (sterile filter) for ambient air ..................................................... 161
22.3 Replacing the CO
2
und O
2
/N
2
gas inlet fine filters (sterile filters) .................................................... 162
22.4 Replacing the CO
2
sensor ............................................................................................................... 163
22.5 Maintenance intervals, service ........................................................................................................ 164
22.6 Simple troubleshooting .................................................................................................................... 165
22.6.1 General ................................................................................................................................. 165
22.6.2 Heating .................................................................................................................................. 166
22.6.3 Gas cylinder pressure too low .............................................................................................. 167
22.6.4 Gas concentration ................................................................................................................. 168
22.6.5 Sterilization ........................................................................................................................... 170
22.6.6 Humidity (CB / CB-UL) .......................................................................................................... 170
22.6.7 Humidity (CBF / CBF-UL) ..................................................................................................... 171
22.6.8 Controller .............................................................................................................................. 171
22.6.9 Open door ............................................................................................................................. 172
22.7 Sending the chamber back to BINDER GmbH ............................................................................... 172
23. DISPOSAL.......................................................................................................... 173
23.1 Disposal of the transport packing .................................................................................................... 173
23.1.1 Outer chamber packing......................................................................................................... 173
23.1.2 Packing inside the chamber and equipment ......................................................................... 173
23.2 Decommissioning ............................................................................................................................ 174
23.3 Disposal of the chamber in the Federal Republic of Germany ....................................................... 174
23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany.......................................................................................................................................... 175
23.5 Disposal of the chamber in non-member states of the EU ............................................................. 176
24. TECHNICAL DESCRIPTION .............................................................................. 177
24.1 Factory calibration and adjustment ................................................................................................. 177
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