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Binder CB 260 User Manual
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Binder CB 260 CO₂ incubator with hot air sterilization, series CB – 220-liter capacity
User Manual - Page 191
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1. Safety
1.1 Personnel Qualification
1.2 Operating manual
1.3 Legal considerations
1.4 Structure of the safety instructions
1.4.1 Signal word panel
1.4.2 Safety alert symbol
1.4.3 Pictograms
1.4.4 Word message panel structure
1.5 Localization / position of safety labels on the chamber
1.6 Type plate
1.7 General safety instructions on installing and operating the CO2 incubator
1.8 Precautions when working with gases
1.9 Precautions when handling gas cylinders
1.10 Intended use
1.11 Foreseeable Misuse
1.12 Residual Risks
1.13 Operating instructions
1.14 Measures to prevent accidents
1.15 CBF / CBF-UL : Resistance of the humidity sensor against harmful substances
2. Chamber description
2.1 Chamber overview
2.2 Inner chamber
2.3 Control panel on the rear of the chamber
2.4 Instrument panel
3. Completeness of delivery, transportation, storage, and installation
3.1 Unpacking, and checking equipment and completeness of delivery
3.2 Guidelines for safe lifting and transportation
3.3 Storage
3.4 Location of installation and ambient conditions
4. Installation and connections
4.1 Shelves
4.2 Permadry⢠water pan
4.3 Connecting the O2 sensor (chamber with O2 control)
4.4 Gas connections
4.4.1 Connection of the CO2 gas cylinder
4.4.2 Connection of the O2 gas cylinder (chamber with O2 control with optional alternative control range 10 up to 95 vol.-% O2)
4.4.3 Connection of the N2 gas cylinder (chamber with O2 control)
4.4.4 Connecting the gas hose to the chamber rear (for CO2, O2, N2)
4.4.5 Gas cylinder connection kits (option)
4.5 CBF / CBF-UL: Water supply and dehumidification
4.5.1 General remarks on water supply
4.5.1.1 Water supply
4.5.1.2 Dehumidification
4.5.1.3 Types of suitable water quality
4.5.1.4 BINDER Pure Aqua Service (option)
4.5.2 Water supply via freshwater bag (standard)
4.5.2.1 Scope of delivery
4.5.2.2 Installation and connection of the freshwater bag
4.5.2.3 Refilling the already installed freshwater bag
4.5.2.4 Replacing the tube connectors for freshwater supply
4.5.3 Water supply via prefilled water bottle (optional humidification water set)
4.5.3.1 Scope of delivery
4.5.3.2 Installation, connection and change of the prefilled water bottle
4.5.4 Water supply via freshwater can (optional water container set)
4.5.4.1 Scope of delivery
4.5.4.2 Installation and connection of the freshwater can (optional water container set)
4.5.4.3 Refilling the freshwater can
4.6 Electrical connection
4.7 Handling and aligning the divided inner door, gas proof (optional equipment)
5. Functional overview of the MB2 chamber controller
5.1 Operating functions in normal display
5.2 Display views: Normal display, program display, chart-recorder display
5.3 Controller icons overview
5.4 Operating modes
5.5 Controller menu structure
5.5.1 Main menu
5.5.2 âSettingsâ submenu
5.5.3 âServiceâ submenu
5.6 Principle of controller entries
5.7 Performance during and after power failures
5.8 Performance when opening the door
6. Start up
6.1 Turning on the chamber
6.2 Controller settings upon start up
6.3 Factory settings
6.4 Equilibration time
6.5 Operating the DuoDoor⢠door lock
6.6 Required gas supply for the chamber with O2 control
6.6.1 Hypoxic control range 0.2 vol.-% up to 20 vol.- % O2 (regular)
6.6.2 Alternative control range 10 vol.-% up to 95 vol.-% (option)
6.6.3 Operation without O2 control
6.7 Activating / deactivating CO2, O2 and humidity control
6.8 CB / CB-UL: Humidity control of the Permadry⢠system
7. Set-point entry in âFixed valueâ operating mode
7.1 Set-point entry through the âSetpointsâ menu
7.2 Direct setpoint entry via Normal display
7.3 Special controller functions via operation lines
7.4 Safety instructions for setting high gas concentrations
8. Timer program: stopwatch function
8.1 Starting a timer program
8.1.1 Performance during program delay time
8.2 Stopping a running timer program
8.2.1 Pausing a running timer program
8.2.2 Cancelling a running timer program
8.3 Performance after the end of the program
9. Time programs
9.1 Starting an existing time program
9.1.1 Performance during program delay time
9.2 Stopping a running time program
9.2.1 Pausing a running time program
9.2.2 Cancelling a running time program
9.3 Performance after the end of the program
9.4 Creating a new time program
9.5 Program editor: program management
9.5.1 Deleting a time program
9.6 Section editor: section management
9.6.1 Add a new program section
9.6.2 Copy and insert or replace a program section
9.6.3 Deleting a program section
9.7 Value entry for a program section
9.7.1 Section duration
9.7.2 Set-point ramp and set-point step
9.7.3 Special controller functions via operation lines
9.7.4 Setpoint entry
9.7.5 Tolerance range
9.7.6 Repeating one or several sections within a time program
9.7.7 Saving the time program
10. Week programs
10.1 Starting an existing week program
10.2 Cancelling a running week program
10.3 Creating a new week program
10.4 Program editor: program management
10.4.1 Deleting a week program
10.5 Section editor: section management
10.5.1 Add a new program section
10.5.2 Copy and insert or replace a program section
10.5.3 Deleting a program section
10.6 Value entry for a program section
10.6.1 Set-point ramp and set-point step modes
10.6.2 Weekday
10.6.3 Start time
10.6.4 Setpoint entry
10.6.5 Special controller functions via operation lines
11. Notification and alarm functions
11.1 Notification and alarm messages overview
11.1.1 Notifications
11.1.2 Alarm messages
11.2 State of alarm
11.3 Resetting an alarm, list of active alarms
11.4 Activating / deactivating the audible alarm (alarm buzzer)
11.5 Tolerance range settings and alarm delay times
11.6 Zero-voltage relay alarm output
12. Temperature safety devices
12.1 Over temperature protective device (class 1)
12.2 Overtemperature safety controller class 3.1
12.2.1 Safety controller modes
12.2.2 Setting the safety controller
12.2.3 Message and measures in the state of alarm
12.2.4 Function check
13. User management
13.1 Authorization levels and password protection
13.2 Log in
13.3 Log out
13.4 User change
13.5 Password assignment and password change
13.5.1 Password change
13.5.2 Deleting the password for an individual authorization level
13.5.3 New password assignment for âserviceâ or âadminâ authorization level when the password function was deactivated
13.6 Activation code
14. General controller settings
14.1 Selecting the controllerâs menu language
14.2 Setting date and time
14.3 Selecting the temperature unit
14.4 Display configuration
14.4.1 Adapting the display parameters
14.4.2 Touchscreen calibration
14.5 Network and communication
14.5.1 Serial RS485 interface (available via BINDER INDIVIDUAL Customized Solutions)
14.5.2 Ethernet
14.5.2.1 Configuration
14.5.2.2 Display of MAC address
14.5.3 Web server
14.5.4 E-Mail
14.6 USB menu: Data transfer via USB interface
15. General information
15.1 Service contact page
15.2 Current operating parameters
15.3 Event list
15.4 Technical chamber information
16. Chart recorder display
16.1 Views
16.1.1 Show and hide legend
16.1.2 Switch between legend pages
16.1.3 Show and hide specific indications
16.1.4 History display
16.2 Setting the parameters
17. CBF / CBF-UL: Humidification system
17.1 Function of the humidifying system
17.2 Function of the dehumidifying system
18. Options
18.1 APT-COM⢠4 Multi Management Software (option)
18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option)
18.3 Quick sample access (option)
18.4 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions)
18.5 Analog outputs for temperature and CO2 (option)
18.6 Data Logger kits (option)
18.7 Access port for extra-low voltage (option)
18.8 BINDER Gas Supply Service â External bottle changer for CO2, N2 or O2 (option)
18.9 Stands
18.9.1 Stacking stand (option)
18.9.2 Base on castors (option)
18.10 Water supply sets (optional for CBF / CBF-UL)
18.10.1 Tubular bag set (option)
18.10.2 Humidification water set (option)
18.10.3 Water container set (option)
19. Reference measurements
19.1 CO2 reference measuring
19.1.1 Measuring CO2 concentration indirectly via the pH of the cell medium
19.1.2 Measuring CO2 directly via chemical indicator tubes
19.1.3 Measuring CO2 directly with an electronic infrared measuring device
19.2 Temperature reference measurement
20. Avoiding microbial contamination
20.1 Cells and media
20.2 Laboratory conditions / equipment around the incubator
20.3 Working and behavior in the lab
20.4 Chamber design and equipment of the CO2 incubator
20.5 Handling the CO2 incubator
21. Cleaning, decontamination / disinfection, and sterilization
21.1 Cleaning
21.2 Decontamination / chemical disinfection of the chamber
21.2.1 Disinfection of the CO2 sensor
21.2.2 CBF / CBF-UL: Disinfection of components of the fresh water supply system
21.3 Hot-air sterilization
21.3.1 Overview
21.3.2 Preparation for a hot-air sterilization
21.3.3 Starting the sterilization cycle and running the hot-air sterilization
21.3.4 Prematurely terminating the sterilization cycle
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective sterilization
21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the cooling-down phase: successful sterilization
21.3.5 Completing the entire sterilization cycle
22. Maintenance and service, troubleshooting, repair, testing
22.1 General information, personnel qualification
22.2 CBF / CBF-UL: Replacing components of the humidifying / dehumidifying system
22.2.1 Replacing the silicone tube of the fresh water supply system
22.2.2 Replacing the pump head of the fresh water supply system
22.2.3 Replacing the gas fine filter (sterile filter) for ambient air
22.3 Replacing the CO2 und O2/N2 gas inlet fine filters (sterile filters)
22.4 Replacing the CO2 sensor
22.5 Maintenance intervals, service
22.6 Simple troubleshooting
22.6.1 General
22.6.2 Heating
22.6.3 Gas cylinder pressure too low
22.6.4 Gas concentration
22.6.5 Sterilization
22.6.6 Humidity (CB / CB-UL)
22.6.7 Humidity (CBF / CBF-UL)
22.6.8 Controller
22.6.9 Open door
22.7 Sending the chamber back to BINDER GmbH
23. Disposal
23.1 Disposal of the transport packing
23.1.1 Outer chamber packing
23.1.2 Packing inside the chamber and equipment
23.2 Decommissioning
23.3 Disposal of the chamber in the Federal Republic of Germany
23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of Germany
23.5 Disposal of the chamber in non-member states of the EU
24. Technical description
24.1 Factory calibration and adjustment
24.2 Over current protection
24.3 Definition of usable volume
24.4 Technical data
24.5 Equipment and Options (extract)
24.6 Accessories and spare parts (extract)
24.7 Important conversion data for non-SI units
24.8 Conversion table for gas inlet pressures, bar â psi
24.9 Dimensions
24.9.1 Chamber size 56
24.9.2 Chamber size 170
24.9.3 Chamber size 260
25. Certificates and declarations of conformity
25.1 EU Declaration of conformity for CB
25.2 EU Declaration of conformity for CBF
26. Contamination clearance certificate
26.1 For chambers located outside the USA and Canada
26.2 For chambers in the USA and Canada
Page 191/197
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Contents
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CB / CB
-
UL
, CBF
/
CBF
-
UL
(E7)
07/2021
Page 191
/
1
97
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File type: PDF
File name: 29621952_cb-170.pdf
File size: 10.72 MB
File Language: English
Pages: 197
Author: Binder
Published: 2021-08-19
Updated: 2023-05-22
Download File
Table of Contents
×
1. Safety
8
1.1 Personnel Qualification
8
1.2 Operating manual
8
1.3 Legal considerations
8
1.4 Structure of the safety instructions
9
1.4.1 Signal word panel
9
1.4.2 Safety alert symbol
9
1.4.3 Pictograms
10
1.4.4 Word message panel structure
11
1.5 Localization / position of safety labels on the chamber
11
1.6 Type plate
12
1.7 General safety instructions on installing and operating the CO2 incubator
13
1.8 Precautions when working with gases
15
1.9 Precautions when handling gas cylinders
17
1.10 Intended use
18
1.11 Foreseeable Misuse
19
1.12 Residual Risks
20
1.13 Operating instructions
21
1.14 Measures to prevent accidents
22
1.15 CBF / CBF-UL : Resistance of the humidity sensor against harmful substances
23
2. Chamber description
24
2.1 Chamber overview
25
2.2 Inner chamber
26
2.3 Control panel on the rear of the chamber
27
2.4 Instrument panel
28
3. Completeness of delivery, transportation, storage, and installation
28
3.1 Unpacking, and checking equipment and completeness of delivery
28
3.2 Guidelines for safe lifting and transportation
29
3.3 Storage
29
3.4 Location of installation and ambient conditions
30
4. Installation and connections
33
4.1 Shelves
33
4.2 Permadry⢠water pan
34
4.3 Connecting the O2 sensor (chamber with O2 control)
35
4.4 Gas connections
35
4.4.1 Connection of the CO2 gas cylinder
36
4.4.2 Connection of the O2 gas cylinder (chamber with O2 control with optional alternative control range 10 up to 95 vol.-% O2)
37
4.4.3 Connection of the N2 gas cylinder (chamber with O2 control)
38
4.4.4 Connecting the gas hose to the chamber rear (for CO2, O2, N2)
39
4.4.5 Gas cylinder connection kits (option)
41
4.5 CBF / CBF-UL: Water supply and dehumidification
42
4.5.1 General remarks on water supply
42
4.5.1.1 Water supply
42
4.5.1.2 Dehumidification
42
4.5.1.3 Types of suitable water quality
42
4.5.1.4 BINDER Pure Aqua Service (option)
43
4.5.2 Water supply via freshwater bag (standard)
43
4.5.2.1 Scope of delivery
43
4.5.2.2 Installation and connection of the freshwater bag
44
4.5.2.3 Refilling the already installed freshwater bag
45
4.5.2.4 Replacing the tube connectors for freshwater supply
46
4.5.3 Water supply via prefilled water bottle (optional humidification water set)
47
4.5.3.1 Scope of delivery
47
4.5.3.2 Installation, connection and change of the prefilled water bottle
47
4.5.4 Water supply via freshwater can (optional water container set)
49
4.5.4.1 Scope of delivery
50
4.5.4.2 Installation and connection of the freshwater can (optional water container set)
50
4.5.4.3 Refilling the freshwater can
52
4.6 Electrical connection
53
4.7 Handling and aligning the divided inner door, gas proof (optional equipment)
54
5. Functional overview of the MB2 chamber controller
55
5.1 Operating functions in normal display
56
5.2 Display views: Normal display, program display, chart-recorder display
57
5.3 Controller icons overview
58
5.4 Operating modes
60
5.5 Controller menu structure
61
5.5.1 Main menu
62
5.5.2 âSettingsâ submenu
63
5.5.3 âServiceâ submenu
63
5.6 Principle of controller entries
64
5.7 Performance during and after power failures
64
5.8 Performance when opening the door
65
6. Start up
65
6.1 Turning on the chamber
65
6.2 Controller settings upon start up
66
6.3 Factory settings
67
6.4 Equilibration time
67
6.5 Operating the DuoDoor⢠door lock
68
6.6 Required gas supply for the chamber with O2 control
68
6.6.1 Hypoxic control range 0.2 vol.-% up to 20 vol.- % O2 (regular)
68
6.6.2 Alternative control range 10 vol.-% up to 95 vol.-% (option)
68
6.6.3 Operation without O2 control
69
6.7 Activating / deactivating CO2, O2 and humidity control
69
6.8 CB / CB-UL: Humidity control of the Permadry⢠system
70
7. Set-point entry in âFixed valueâ operating mode
71
7.1 Set-point entry through the âSetpointsâ menu
72
7.2 Direct setpoint entry via Normal display
73
7.3 Special controller functions via operation lines
74
7.4 Safety instructions for setting high gas concentrations
75
8. Timer program: stopwatch function
76
8.1 Starting a timer program
76
8.1.1 Performance during program delay time
76
8.2 Stopping a running timer program
77
8.2.1 Pausing a running timer program
77
8.2.2 Cancelling a running timer program
77
8.3 Performance after the end of the program
77
9. Time programs
78
9.1 Starting an existing time program
78
9.1.1 Performance during program delay time
79
9.2 Stopping a running time program
79
9.2.1 Pausing a running time program
79
9.2.2 Cancelling a running time program
79
9.3 Performance after the end of the program
79
9.4 Creating a new time program
80
9.5 Program editor: program management
80
9.5.1 Deleting a time program
81
9.6 Section editor: section management
82
9.6.1 Add a new program section
83
9.6.2 Copy and insert or replace a program section
83
9.6.3 Deleting a program section
84
9.7 Value entry for a program section
85
9.7.1 Section duration
85
9.7.2 Set-point ramp and set-point step
86
9.7.3 Special controller functions via operation lines
88
9.7.4 Setpoint entry
89
9.7.5 Tolerance range
89
9.7.6 Repeating one or several sections within a time program
90
9.7.7 Saving the time program
91
10. Week programs
92
10.1 Starting an existing week program
92
10.2 Cancelling a running week program
92
10.3 Creating a new week program
93
10.4 Program editor: program management
94
10.4.1 Deleting a week program
95
10.5 Section editor: section management
96
10.5.1 Add a new program section
97
10.5.2 Copy and insert or replace a program section
97
10.5.3 Deleting a program section
98
10.6 Value entry for a program section
98
10.6.1 Set-point ramp and set-point step modes
98
10.6.2 Weekday
99
10.6.3 Start time
99
10.6.4 Setpoint entry
100
10.6.5 Special controller functions via operation lines
100
11. Notification and alarm functions
101
11.1 Notification and alarm messages overview
101
11.1.1 Notifications
101
11.1.2 Alarm messages
102
11.2 State of alarm
104
11.3 Resetting an alarm, list of active alarms
104
11.4 Activating / deactivating the audible alarm (alarm buzzer)
105
11.5 Tolerance range settings and alarm delay times
105
11.6 Zero-voltage relay alarm output
107
12. Temperature safety devices
108
12.1 Over temperature protective device (class 1)
108
12.2 Overtemperature safety controller class 3.1
108
12.2.1 Safety controller modes
109
12.2.2 Setting the safety controller
109
12.2.3 Message and measures in the state of alarm
110
12.2.4 Function check
110
13. User management
111
13.1 Authorization levels and password protection
111
13.2 Log in
114
13.3 Log out
115
13.4 User change
115
13.5 Password assignment and password change
116
13.5.1 Password change
116
13.5.2 Deleting the password for an individual authorization level
118
13.5.3 New password assignment for âserviceâ or âadminâ authorization level when the password function was deactivated
119
13.6 Activation code
120
14. General controller settings
121
14.1 Selecting the controllerâs menu language
121
14.2 Setting date and time
121
14.3 Selecting the temperature unit
123
14.4 Display configuration
123
14.4.1 Adapting the display parameters
123
14.4.2 Touchscreen calibration
124
14.5 Network and communication
125
14.5.1 Serial RS485 interface (available via BINDER INDIVIDUAL Customized Solutions)
125
14.5.2 Ethernet
126
14.5.2.1 Configuration
126
14.5.2.2 Display of MAC address
127
14.5.3 Web server
127
14.5.4 E-Mail
128
14.6 USB menu: Data transfer via USB interface
129
15. General information
130
15.1 Service contact page
130
15.2 Current operating parameters
130
15.3 Event list
131
15.4 Technical chamber information
131
16. Chart recorder display
132
16.1 Views
132
16.1.1 Show and hide legend
132
16.1.2 Switch between legend pages
132
16.1.3 Show and hide specific indications
133
16.1.4 History display
133
16.2 Setting the parameters
136
17. CBF / CBF-UL: Humidification system
137
17.1 Function of the humidifying system
137
17.2 Function of the dehumidifying system
138
18. Options
138
18.1 APT-COM⢠4 Multi Management Software (option)
138
18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option)
138
18.3 Quick sample access (option)
139
18.4 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions)
140
18.5 Analog outputs for temperature and CO2 (option)
141
18.6 Data Logger kits (option)
142
18.7 Access port for extra-low voltage (option)
142
18.8 BINDER Gas Supply Service â External bottle changer for CO2, N2 or O2 (option)
143
18.9 Stands
144
18.9.1 Stacking stand (option)
144
18.9.2 Base on castors (option)
144
18.10 Water supply sets (optional for CBF / CBF-UL)
144
18.10.1 Tubular bag set (option)
144
18.10.2 Humidification water set (option)
144
18.10.3 Water container set (option)
144
19. Reference measurements
145
19.1 CO2 reference measuring
145
19.1.1 Measuring CO2 concentration indirectly via the pH of the cell medium
145
19.1.2 Measuring CO2 directly via chemical indicator tubes
146
19.1.3 Measuring CO2 directly with an electronic infrared measuring device
146
19.2 Temperature reference measurement
146
20. Avoiding microbial contamination
147
20.1 Cells and media
147
20.2 Laboratory conditions / equipment around the incubator
147
20.3 Working and behavior in the lab
147
20.4 Chamber design and equipment of the CO2 incubator
148
20.5 Handling the CO2 incubator
149
21. Cleaning, decontamination / disinfection, and sterilization
150
21.1 Cleaning
150
21.2 Decontamination / chemical disinfection of the chamber
152
21.2.1 Disinfection of the CO2 sensor
153
21.2.2 CBF / CBF-UL: Disinfection of components of the fresh water supply system
153
21.3 Hot-air sterilization
154
21.3.1 Overview
154
21.3.2 Preparation for a hot-air sterilization
155
21.3.3 Starting the sterilization cycle and running the hot-air sterilization
155
21.3.4 Prematurely terminating the sterilization cycle
157
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective sterilization
157
21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the cooling-down phase: successful sterilization
158
21.3.5 Completing the entire sterilization cycle
159
22. Maintenance and service, troubleshooting, repair, testing
160
22.1 General information, personnel qualification
160
22.2 CBF / CBF-UL: Replacing components of the humidifying / dehumidifying system
161
22.2.1 Replacing the silicone tube of the fresh water supply system
161
22.2.2 Replacing the pump head of the fresh water supply system
161
22.2.3 Replacing the gas fine filter (sterile filter) for ambient air
161
22.3 Replacing the CO2 und O2/N2 gas inlet fine filters (sterile filters)
162
22.4 Replacing the CO2 sensor
163
22.5 Maintenance intervals, service
164
22.6 Simple troubleshooting
165
22.6.1 General
165
22.6.2 Heating
166
22.6.3 Gas cylinder pressure too low
167
22.6.4 Gas concentration
168
22.6.5 Sterilization
170
22.6.6 Humidity (CB / CB-UL)
170
22.6.7 Humidity (CBF / CBF-UL)
171
22.6.8 Controller
171
22.6.9 Open door
172
22.7 Sending the chamber back to BINDER GmbH
172
23. Disposal
173
23.1 Disposal of the transport packing
173
23.1.1 Outer chamber packing
173
23.1.2 Packing inside the chamber and equipment
173
23.2 Decommissioning
174
23.3 Disposal of the chamber in the Federal Republic of Germany
174
23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of Germany
175
23.5 Disposal of the chamber in non-member states of the EU
176
24. Technical description
177
24.1 Factory calibration and adjustment
177
24.2 Over current protection
177
24.3 Definition of usable volume
178
24.4 Technical data
178
24.5 Equipment and Options (extract)
181
24.6 Accessories and spare parts (extract)
182
24.7 Important conversion data for non-SI units
184
24.8 Conversion table for gas inlet pressures, bar â psi
184
24.9 Dimensions
185
24.9.1 Chamber size 56
185
24.9.2 Chamber size 170
186
24.9.3 Chamber size 260
187
25. Certificates and declarations of conformity
188
25.1 EU Declaration of conformity for CB
188
25.2 EU Declaration of conformity for CBF
190
26. Contamination clearance certificate
192
26.1 For chambers located outside the USA and Canada
192
26.2 For chambers in the USA and Canada
195
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