Binder CB 260 CO₂ incubator with hot air sterilization, series CB – 220-liter capacity

User Manual - Page 154

For CB 260.

PDF File Manual, 197 pages, Read Online | Download pdf file

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CB / CB-UL, CBF / CBF-UL (E7) 07/2021 Page 154/197
21.3 Hot-air sterilization
The very first sterilization after operation may cause an odor. This is not a quality defect. We
recommend ventilating well the room during sterilization.
21.3.1 Overview
Heating up
Holding Cooling down Equilibrating
4 hours 6 hours unlimited
Sterilization cycle of 10 hours
Figure 39: Setpoint profile during the sterilization cycle
The chamber can perform an automatically controlled hot-air sterilization cycle. This procedure will take
approx. 10 hours and consists of the following steps:
Heating up phase: The chamber heats up the inner chamber as fast as possible to the sterilization
setpoint temperature
Holding phase: Constant sterilization setpoint temperature
The sterilization setpoint temperature is pre-set in factory to 180 °C / 356 °F. The duration of the heating
up and holding phase is in total 4 hours. This ensures that the effective sterilization temperature is
maintained on all internal surfaces for at least 2 hours. The effectiveness of the sterilization has also
been confirmed by an independent research institute.
Cooling down phase: Programmed duration of 6 hours until 37 °C / 98.6°F is reached
Then follows an equilibration phase: After the 10-hour sterilization cycle the controller changes to fixed
value operation mode and equilibrates to the set-point which has been entered there (e.g., 37 °C /
98.6 °F).
Note: in the case when two chambers are directly stacked on top of each other without using the original
BINDER stacking stand or the BINDER stacking adapter, the exact maintenance of the incubation set
parameters in one of the chambers while carrying out sterilization in the other one cannot be assured.
Therefore, without using the stacking stand or the stacking adapter, safe incubation is not possible during
sterilization of the other chamber. We recommend using a stacking stand (chap. 18.9.1).
CO
2
/ O
2
control is deactivated during the sterilization cycle. To activate gas control, the following conditions
are required:
Completing or prematurely terminating the sterilization cycle and confirming the corresponding
information message
Inner chamber temperature below 80 °C
If the temperature exceeds < 80 °C, gas control will remain deactivated even after completing
or prematurely terminating the sterilization cycle.
Only after the temperature has decreased below 80 °C, gas control will turn on again.
37 °C
180 °C
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