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CB / CB-UL, CBF / CBF-UL (E7) 07/2021 Page 148/197
Examples of sterile working method
• Work "clean-to-dirty", i.e., handle confirmed uncontaminated cells first, unknown or untested cells next,
and lastly, if necessary, cells suspected to be contaminated.
• Perform daily microscopic observations of cultures and specific tests for the bacteria and fungi as part
of a controlled routine. Test cultures for sterility before starting work.
• Keep working surfaces clean. Immediately wipe spilled liquids with alcohol solutions.
• No mouth contact on pipettes.
• Never work on top of open sterile containers.
20.4 Chamber design and equipment of the CO
2
incubator
The design concepts behind the CO
2
incubator considerably reduce the risk of contamination. Among them
are:
Even surfaces for easy manual cleaning
• The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from a
single piece, polished (suitable for pharmaceutical work) and has no welds or inaccessible corners. The
hinges and the seal of the inner glass door are glued from the outside, which also aids cleaning of the
inner chamber.
Removable parts for cleaning and autoclaving
• The shelves are easily removed without screws. It is possible to autoclave the shelves. But this is
generally not necessary because they can remain inside the chamber during sterilization.
Door gasket
• The inner door gasket is removable and autoclavable.
Gas fine filter
• The incoming gas used in the operation passes through a fine filter (aseptic filter, filtration efficiency
99.99 %, particle size 0.45 µm) with a high filtration efficiency, which can also filter the smallest particles.
CO
2
measuring system in the inner chamber
• The CO
2
sensor can be hot-air sterilized inside the chamber.
O
2
measuring system in the inner chamber (chamber with O
2
control)
• The O
2
sensor can be hot-air sterilized inside the chamber.
Humidity measuring system in the inner chamber (CBF / CBF-UL)
• The humidity sensor can be hot-air sterilized inside the chamber.
Condensation prevention
• Condensation in the inner chamber represents a particular risk of contamination. The Permadry™
system developed by BINDER is an effective and easy way to ensure high humidity (≥95 % r.h.) inside
the chamber without any condensation forming on the inner surfaces.
Hot air sterilization
• The heating system of the CO
2
incubator permits hot-air auto-sterilization at a setpoint of 180 °C / 356 F.
Thus, the effective sterilization temperature is maintained for at least two hours on all internal surfaces,
resulting in sterilization of the entire inner chamber.
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