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CB-S / CB-S-UL (E7) 06/2020 page 81/119
CO
2
measuring system in the inner chamber
• You can remove the CO
2
sensor from the inner chamber by hand for disinfection (chap. 22.3).
Condensation prevention
• Condensation in the inner chamber represents a particular risk of contamination. The humidifying sys-
tem with water pan with integrated condensation point developed by BINDER is an effective and easy
way to ensure high humidity (95 +/- 2% r.h.) inside the incubator without any condensation forming on
the inner surfaces.
Hot air sterilization
• The heating system of the chamber permits hot-air auto-sterilization (chap. 23). Thus, a temperature of
180 °C / 356°F is maintained for a 30-minute period on all internal surfaces during its cycle, resulting
in sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines
regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia.
21.5 Handling the CO
2
incubator
Any manipulation of the CO
2
incubator involves some contamination risks, from installation to opening of
the doors and regular cleaning.
Installation away from sources of contamination
• Do not place the CO
2
incubator on the floor or close to windows and doors. Use the optional stand, if
appropriate.
Reduce the periods in which the door is open.
• Do not open the door too frequently.
• Placing items in order inside the incubator results in shorter door opening times.
Water pan
• Fill the water pan with distilled, sterilized water (chap. 4.3). Never use ion exchange water; ion ex-
changers are propagation sites for bacteria.
• Clean and refill the pans 2 to 3 times a week. For evacuation, remove the water pan. It is autoclava-
ble.
• If desired, you can add microbiologically inhibiting substances such as copper chips, copper sulphate
or ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.
Avoiding condensation caused by ambient conditions
• Ambient room conditions have an effect on condensation inside the incubator, which can be caused by
insufficient wall clearances, preventing even dissipation of heat, air movement or direct sunlight. If the
temperature distribution inside the chamber becomes uneven, condensation may form on the cooler
surfaces.
• Maintain distances from the wall: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.
• Do not place the chamber in front of a window. No direct sunlight. No air movement.
• Permissible ambient temperature range for operation: +18 °C / 64.4°F to +30 °C / 86°F. Ideal ambient
temperature: at least 7 degrees below the intended working temperature. E.g., working temperature
37 °C / 98.6°F = ambient temperature 30 °C / 86°F and less.
• The incubator should be precisely calibrated / adjusted.
Regular cleaning, decontamination, and sterilization
• Clean the shelves, glass door, gaskets, and inner chamber weekly (for cleaning see chap. 22.1, for
decontamination see chap. 22.2). You can clean the shelves in a laboratory dishwasher and, if need-
ed, individually autoclave them.
• Regularly use the hot air sterilization function (chap. 23) following cleaning. The shelves and the emp-
tied water pan can remain inside the incubator during this operation.
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