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CB-S / CB-S-UL (E7) 06/2020 page 19/119
2. Chamber description
The CO
2
incubators CB-S / CB-S-UL series were produced with great care using the latest tools for de-
velopment and production. They can be operated in a temperature range from 6 °C / 10.8 °F above ambi-
ent temperature up to +50 °C / 122°F and a CO
2
range of 0 vol.-% up to 20 vol.-%. The chambers are
equipped with a microprocessor controller for temperature and CO
2
levels and a digital display accurate
to one-tenth of a degree resp. 0.1 vol.-%. They are available in different voltages.
Material: The inner chamber, the pre-heating chamber and the inside of the doors are all made of stain-
less steel V2A (German material no. 1.4301, US equivalent AISI 304). The inner surfaces are smooth and
therefore easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharma-
ceutical applications) and has no welds or inaccessible corners. The hinges and the seal of the inner
glass door are glued from the outside to aid cleaning of the inner chamber. When operating the chamber
at high temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the
metallic surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harm-
less and will in no way impair the function or quality of the chamber.
The perforated shelves are also made of stainless steel (German material no. 1.4016, US equivalent AISI
430). You can insert a maximum of 6 (CB-S / CB-S-UL 170) bzw. 8 (CB-S / CB-S-UL 260) shelves.
The housing is RAL 7035 powder-coated. All corners and edges are also completely coated. The stand-
ard chamber door is hinged right. The chamber is optionally available with the door hinged left.
Sterilization: The heating system of the chamber permits hot-air auto-sterilization at 190 °C / 374 °F.
Thus, a temperature of at least 180 °C / 356 °F is maintained on all internal surfaces, resulting in steriliza-
tion of the entire inner chamber.
Safety: Thanks to the standard safety device (class 3.1 according to DIN 12880:2007), the set tempera-
ture is maintained in case of failure.
An error diagnostics system monitors the chamber functions and generates audible and visual warning
and alarm messages. The door is monitored for being closed.
The controller provides access control by combining password protection with different authorization lev-
els.
CO
2
system: A highly precise, drift-free CO
2
infrared measuring system in combination with the perma-
nent mixture of CO
2
gas through a special proprietary gas mixing head developed by BINDER allows
precise and constant CO
2
concentrations for long periods. This creates optimum growth conditions for
cultures. The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also
filters the smallest particles.
The CO
2
sensor can be removed from the inner chamber by hand and cleaned with suitable detergents if
needed.
Fast reaction times, maximum accuracy and selectivity characterize the CO
2
measuring procedure of the
chamber. The accuracy of the CO
2
measuring system is based on an infrared measuring cell with NDIR
(non-dispersive infrared) sensor, which continuously regulates to a reference value. Therefore, disturb-
ance variables and aging phenomena in the measuring system are almost completely eliminated, so that
this measuring system, in contrast to other measuring procedures, remains practically drift-free between
calibrations and is entirely selective for CO
2
.
The CO
2
measuring cell contains a measuring section inside, in which the absorption of infrared light
depends on the number of CO
2
molecules in the beam path. This number of CO
2
molecules changes with
the ambient pressure in relation to a constant volume. The distances between the molecules are conse-
quently pressure-dependent. The collision frequency of the IR-beam with CO
2
molecules increases there-
fore by increasing pressure. For this reason, the ambient pressure must be compensated in order to cor-
rect the display reading of the CO
2
concentration in vol.-%. This is achieved by entering the altitude of the
site above the sea (chap. 6.4).
Controller: The efficient RD4 chamber controller is equipped with a multitude of operating functions, in
addition to recorder and alarm functions. Set-point entry is easily accomplished accurate to one-tenth of a
degree resp. 0.1 vol.-% directly via the chamber controller and is also possible directly with a computer
via Intranet in connection with the APT-COM™ 4 Multi Management Software (option, chap. 20.4). The
controller provides password protection for the setting menus. An error diagnostics system generates
audible and visual alarm messages.
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