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CB-S / CB-S-UL (E7) 06/2020 page 79/119
20.6 Analog outputs for temperature and CO
2
(option)
With this option, the CO
2
incubator is equipped with analog outputs 4-20 mA for temperature and CO
2
.
These outputs allow transmitting data to external data registration systems or devices.
The connection is realized as a DIN socket at the rear of the CO
2
incubator as follows:
ANALOG OUTPUT 4-20 mA DC
PIN 1: Temperature –
PIN 2: Temperature +'
PIN 3: CO
2
–
PIN 4: CO
2
+
CO
2
range: 0 vol.-% up to 20 vol.-%
Temperature range: 0 °C / 32°F up to +200 °C / 392°F
A suitable DIN plug is enclosed.
Figure 20: Pin allocation of DIN socket for option analog outputs
21. Avoiding microbial contamination
The main types of microbial contaminants in cell and tissue culture are bacteria, fungi, yeast, mycoplas-
ma, and viruses. This chapter gives an overview of potential sources of contamination and precautions
and measures to eliminate them.
21.1 Cells and media
• Primary cultures from the original tissue
• Cells/cell lines from unknown sources or from cell banks: Use only cells of known and tested origin.
Monitoring and routine screening of new cultures.
• Media and sera: Use only sera of known and tested origin (mycoplasma free, e.g., UV or γ radiated).
• Virus suspensions, antibody solutions etc. Use only reagents of known and tested origin.
• Laboratory instruments, media and reagents, which were exposed to possible contaminated cultures
must be sterilized / autoclaved / disposed.
• Antibiotics in the cell culture media may prevent bacteria detection: Use antibiotics selectively and
economically.
21.2 Laboratory conditions / equipment around the CO
2
incubator
Possible sources of contamination in the cell culture lab are airborne germs, lab equipment, building fea-
tures, and the lab personnel.
• Keep pipettes and instruments sterile after autoclaving.
• Bio safety cabinets (laminar air flow) should have a minimum of items apart from aspirator tube and
burner. Items shall be positioned within easy reach and separate from each other. Disinfect surfaces
with an alcohol-based disinfectant before and after use, clean the space underneath the bench, and
carry out regular sterility tests of the filters.
• Regular cleaning / disinfection of laboratory equipment such as a centrifuge, microscope, water bath,
refrigerator, and telephone.
• No equipment should be placed on the floor.
• Rough or humid walls are not suitable.
• Identify leaking doors and windows and make them airtight.
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