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UF V (E3) 10/2020 Page 83/119
• Make sure that operating instructions on the connection and exchange of the pressurized gas cylinders
are attached in the vicinity of the pressurized gas cylinders. The instructions shall contain all required
safety-related information in an intelligible form.
• Laboratories in which compressed gas cylinders are installed must be labeled with
the warning sign W019 “Warning of gas cylinders”.
• In areas with increased risk of fire, if possible set up gas cylinders outside the rooms and install fix
tubing.
Carbon dioxide (CO
2
) in high concentrations (> 4 Vol.-%) is hazardous to health. It is colorless and almost
odorless and therefore practically imperceptible. Escaping CO
2
gas is heavier than air and accumulates at
the ground or possibly in lower-lying parts of the building. There is danger of suffocation and poisoning.
Hazards due to uncontrolled gas release must be effectively avoided.
DANGER
Danger of suffocation and poisoning by high concentration of CO
2
(> 4 Vol.-%).
Death by suffocation.
∅ Operate the CO
2
emergency cooling only in a well-ventilated room.
Ensure technical ventilation measures. Install a suitable conduct at the CO2 discharge
outlet of the emergency cooling system.
Observe the relevant regulations for handling CO2.
Close the CO2 supply when decommissioning the chamber.
When installing pressurized CO
2
cylinders take into account the structure of the building.
• The installation site must be in a well-ventilated area (natural ventilation). For rooms with a with a floor
area of ≤ 12 m
2
with solid walls without openings on all sides there are further regulations (max. two 14-
liter CO
2
cylinders, Label “suffocation” and prohibition to close the door after entering).
• The installation site must be technically ventilated (technical ventilation) with a CO
2
alarm system (gas
warning device)
We strongly recommend continuous monitoring of the CO
2
concentration in the ambient air of the CO
2
emergency cooling. It must be permanently ensured that the occupational exposure limit OEL (formerly
maximum permitted workplace concentration) of 0.5 Vol.-% CO
2
is not exceeded.
21.2 Operating the CO
2
emergency cooling system
When operating the CO
2
emergency cooling system, the freezer’s interior is flooded with CO
2
. CO
2
in high
concentrations (> 4 vol.-%) is hazardous to health. It is colorless and almost odorless and therefore practi-
cally imperceptible. Operate the CO
2
emergency cooling system only in a well-ventilated room. Released
CO
2
gas muss must be safely led out via good room ventilation or a suitable connection to an exhaust
system and through a conduct at the CO
2
discharge outlet (14) on the rear of the emergency cooling sys-
tem. Observe the occupational exposure limit for CO
2
set by the national authorities. We recommend in-
stalling a CO
2
warning system.
Even when CO
2
or systems operated with CO
2
are handled carefully and appropriately, a residual risk
remains, which can lead to life-threatening situations under certain circumstances. Therefore, we strongly
recommend continuous monitoring of CO
2
concentration in the ambient air of CO
2
emergency cooling. It
must be ensured permanently that the maximum permissible occupational exposure limit for CO
2
(0.5 vol -
% CO
2
for Germany) is not exceeded.
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