Loading ...
Loading ...
Loading ...
88
RNeasy Mini Handbook 10/2019
4. Centrifuge for 10 min at maximum speed in a benchtop centrifuge. Discard the
supernatant and air-dry the pellet.*
5. Optional: Wash the pellet with 100 µl ice-cold ethanol and air-dry.
Do not overdry the pellet as this may make resuspension more difficult.
6. Resuspend the pellet in the buffer for your downstream application.
Sodium dodecyl sulfate (SDS) causes guanidine salts to precipitate. In case the pellet
contains traces of guanidine thiocyanate, load the sample onto an SDS-PAGE gel
immediately after heating for 7 min at 95°C.
* Supernatant contains guanidine thiocyanate and is therefore not compatible with bleach. See page 6 for safety
information.
Loading ...
Loading ...
Loading ...