RNEASY KITS User Manual

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RNeasy Mini Handbook 10/2019

Introduction

The

RNeasy Mini Handbook

provides protocols for use with the following kits:

 RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and

yeast, and for cleanup of RNA from crude RNA preps and enzymatic reactions (e.g.,

DNase digestion, proteinase digestion, RNA ligation, and labeling reaction)

 RNeasy Protect Mini Kit — for immediate stabilization of RNA in harvested animal tissues

and subsequent purification of total RNA

 RNeasy Plant Mini Kit — for purification of total RNA from plant cells and tissues and

filamentous fungi

The RNeasy Mini Kit can also be used to purify total RNA from bacteria. In this case, we

strongly recommend using the kit in combination with RNAprotect

Bacteria Reagent (available

separately), which provides in vivo stabilization of RNA in bacteria to ensure reliable gene

expression analysis. Various protocols for stabilizing and purifying RNA from different

bacteria species are included in the

RNAprotect Bacteria Reagent Handbook

. The RNeasy

Mini Kit and RNAprotect Bacteria Reagent can also be purchased together as the RNeasy

Protect Bacteria Mini Kit. For ordering information, see page 91. It is also possible to use the

RNeasy Mini Kit to purify cytoplasmic RNA from animal cells. The protocol can be downloaded

at www.qiagen.com/resources/RNeasyMini

The RNeasy Kits are designed to purify RNA from small amounts of starting material. They

provide a fast and simple method for preparing up to 100 µg total RNA per sample. The

purified RNA is ready for use in downstream applications such as:

 RT-PCR and real-time RT-PCR

 Differential display

 cDNA synthesis

 Northern, dot, and slot blot analyses

 Primer extension

 Poly A+ RNA selection

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User Manual - Page 8