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RNeasy Mini Handbook 10/2019
11
RNA stabilization using RNAprotect technology
RNA stabilization is an absolute prerequisite for reliable gene expression analysis. Immediate
stabilization of RNA in biological samples is necessary because, directly after harvesting the
samples, changes in the gene expression pattern occur due to specific and nonspecific RNA
degradation as well as to transcriptional induction. Such changes need to be avoided for all
reliable quantitative gene expression analyses, such as microarray analyses, quantitative
RT-PCR, such as TaqMan
®
and LightCycler
®
technology, and other nucleic acid-based
technologies.
The RNeasy Protect Mini Kit is supplied with RNAprotect Tissue Reagent, which represents a
novel technology for the immediate preservation of the gene expression pattern in animal
tissues, enabling reliable gene expression analysis. After harvesting, tissues are immediately
submerged in RNAprotect Tissue Reagent, which rapidly permeates the tissues to stabilize and
protect cellular RNA in situ. The reagent preserves RNA for up to 1 day at 37°C, 7 days at
15–25°C, or 4 weeks at 2–8°C, allowing transportation, storage, and shipping of samples
without ice or dry ice. Alternatively, the samples can be archived at −30 to −15°C or −90 to
−65°C. During storage or transport in RNAprotect Tissue Reagent, even at elevated
temperatures (e.g., room temperature or 37°C), the cellular RNA remains intact and
undegraded. RNAprotect technology allows large numbers of samples to be easily processed
and replaces inconvenient, dangerous, and equipment-intensive methods, such as
snap-freezing of samples in liquid nitrogen, storage at −90 to −65°C, cutting and weighing
on dry ice, or immediate processing of harvested samples.
Note: RNAprotect Tissue Reagent is not for stabilization of RNA in animal cells, whole blood,
plasma, or serum.
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