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34
RNeasy Mini Handbook 10/2019
10. Place the RNeasy spin column in a new 1.5 ml collection tube (supplied). Add 30–50 µl
RNAse-free water directly to the spin column membrane. Close the lid gently, and
centrifuge for 1 min at ≥8000 x
g
(≥10,000 rpm) to elute the RNA.
11. If the expected RNA yield is >30 µg, repeat step 10 using another 30–50 µl RNAse-free
water, or using the eluate from step 10 (if high RNA concentration is required). Reuse the
collection tube from step 10.
If using the eluate from step 10, the RNA yield will be 15–30% less than that obtained
using a second volume of RNAse-free water, but the final RNA concentration will be
higher.
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