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RNeasy Mini Handbook 10/2019
19
Important Notes
Determining the amount of starting material
It is essential to use the correct amount of starting material in order to obtain optimal RNA
yield and purity. The maximum amount that can be used is determined by:
The type of sample and its RNA content
The volume of Buffer RLT required for efficient lysis
The RNA binding capacity of the RNeasy spin column
When processing samples containing high amounts of RNA, less than the maximum amount
of starting material shown in Table 1 should be used, so that the RNA binding capacity of the
RNeasy spin column is not exceeded.
Table 1. RNeasy Mini Spin Column specifications
*For larger sample sizes, RNeasy Kits and RNeasy Protect Kits are available in midi and maxi formats. For details, visit
www.qiagen.com/RNA.
Maximum binding capacity 100 µg RNA
Maximum loading volume
700 μl
RNA size distribution
RNA >200 nucleotides
Minimum elution volume
30 μl
Maximum amount of starting material
• Animal cells
1 x 107*
• Animal tissues
30 mg*
• Yeast
5 x 10
7
*
• Plant tissues
100 mg
• Filamentous fungi
100 mg
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