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16
RNeasy Mini Handbook 10/2019
Equipment and Reagents to Be Supplied by User
When working with chemicals, always wear a suitable lab coat, disposable gloves, and
protective goggles. For more information, consult the appropriate safety data sheets (SDSs),
available from the product supplier.
For all protocols
14.3 M β-mercaptoethanol (β-ME) (commercially available solutions are usually 14.3 M)
Sterile, RNAse-free pipette tips
Microcentrifuge (with rotor for 2 ml tubes)
96–100% ethanol*
Disposable gloves
Equipment for sample disruption and homogenization (see page 22). Depending on the
method chosen, one or more of the following are required:
Trypsin and PBS
QIAshredder homogenizer (see ordering information, page 91)
Blunt needle and syringe
Mortar and pestle
TissueLyser (see ordering information, page 91)
Rotor–stator homogenizer
For RNA purification from animal cells
70% ethanol*
For RNA purification from animal cells using vacuum technology
QIAvac 24 (no longer available); QIAvac 24 Plus (cat. no. 19413); QIAvac 6S (cat. no.
19503) with the QIAvac Luer Adapter Set (cat. no. 19541); or other vacuum manifold with
luer connectors and capable of dealing with vacuum pressures of −800 to −900 mbar
* Do not use denatured alcohol, which contains other substances such as methanol or methylethylketone.
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