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RNeasy Mini Handbook 10/2019
43
Procedure
1. Before excising the tissue sample, estimate the volume (or weight) of the sample to be
stabilized in RNAprotect Tissue Reagent.
2. Determine the appropriate volume of RNAprotect Tissue Reagent for preserving the
tissue. At least 10 volumes of the reagent (or approximately 10 µl reagent per 1 mg of
tissue) is required. Pipet the correct amount of reagent into an appropriate collection
vessel.
Note: Be sure to completely submerge the tissue in RNAprotect Tissue Reagent. For
details, see “Important notes about RNAprotect Tissue Reagent”, page 41.
3. Excise the tissue sample from the animal and, if necessary, cut it into slices less than
0.5 cm thick. Perform this step as quickly as possible and proceed immediately to step 4.
Note: For effective RNA stabilization, the tissue sample must be less than 0.5 cm thick.
For details, see “Important notes about RNAprotect Tissue Reagent”, page 41.
4. Completely submerge the tissue piece(s) in the collection vessel containing RNAprotect
Tissue Reagent from step 2.
Note: The tissue sample must be immediately submerged in RNAprotect Tissue Reagent to
protect the RNA.
5. Store the tissue submerged in RNAprotect Tissue Reagent for up to 4 weeks at 2–8°C, up
to 7 days at 15–25°C, or up to 1 day at 37°C.
For archival storage at −30 to −15°C, first incubate the tissue overnight in the reagent at
2–8°C. Then transfer the tissue, in the reagent, to −30 to −15°C for storage.
For archival storage at −90 to −65°C, first incubate the tissue overnight in the reagent at
2–8°C. Then remove the tissue from the reagent, and transfer it to −90 to −65°C for
storage.
Note: Lower temperatures are recommended for longer storage (e.g., 2–8°C for up to
4 weeks instead of 37°C or room temperature; −30 to −15°C or −90 to −65°C for
longer storage).
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