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RNeasy Mini Handbook 10/2019
81
Gel running conditions
Run gel at 5–7 V/cm in 1x FA gel running buffer.
Composition of FA gel buffers
10x FA gel buffer
200 mm 3-[N-morpholino]propanesulfonic acid (MOPS) (free acid)*
50 mm sodium acetate*
10 mm EDTA*
pH to 7.0 with NaOH*
1x FA gel running buffer
100 ml 10x FA gel buffer
20 ml 37% (12.3 M) formaldehyde
880 ml RNAse-free water
5x RNA loading buffer
16 µl saturated aqueous bromophenol blue solution†
‡
80 µl 500 mm EDTA, pH 8.0
720 µl 37% (12.3 M) formaldehyde
2 ml 100% glycerol*
3.084 ml formamide*
4 ml 10 x FA gel buffer
RNAse-free water to 10 ml
Stability: approximately 3 months at 4°C
* When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For
more information, consult the appropriate material safety data sheets (MSDSs), available from the product supplier.
†
When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more
information, consult the appropriate safety data sheets (SDSs), available from the product supplier.
‡
To make a saturated solution, add solid bromophenol blue to distilled water. Mix and continue to add more
bromophenol blue until no more will dissolve. Centrifuge to pellet the undissolved powder, and carefully pipet the
saturated supeRNAtant
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