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CB / CB-UL, CBF / CBF-UL (E7) 07/2021 Page 149/197
20.5 Handling the CO
2
incubator
Any manipulation of the CO
2
incubator involves some contamination risks, from installation to opening the
doors and regular cleaning.
Installation away from sources of contamination
• Do not place the chamber on the floor or close to windows and doors. Use the optional stand, if
appropriate.
Reduce the periods in which the door is open
• Do not open the door too frequently.
• Placing items in order inside the chamber results in shorter door opening times.
Permadry™ water pan
• Fill the outer pan with distilled, sterilized water (chap. 4.2). Never use ion exchange water; ion
exchangers are propagation sites for bacteria.
• Clean and refill the pans 2 to 3 times a week. For evacuation, remove the Permadry™ water pan. It is
autoclavable.
• If desired, you can add microbiologically inhibiting substances as copper chips, copper sulfate or
ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.
Avoiding condensation caused by ambient conditions
Ambient room conditions have an effect on condensation inside the chamber, which can be caused by
insufficient wall clearances, preventing even dissipation of heat, air movement or direct sunlight. If the
temperature distribution inside the chamber becomes uneven, condensation may form on the cooler
surfaces.
• Maintain distances from the wall: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in
• Do not place the chamber in front of a window. No direct sunlight. No air movement.
• Permissible ambient temperature range for operation: +18 °C / 64.4°F to +30 °C / 86°F.
Ideal ambient temperature: by at least 7 °C / 12.6 °F below the intended working temperature. E.g.,
working temperature 37 °C / 98.6 °F = ambient temperature 30 °C / 86 °F and less.
• The chamber should be calibrated / precisely adjusted.
• Let BINDER Service adjust the setting of the door heating in reaction to critical ambient conditions.
Regular cleaning, decontamination and sterilization
• Clean the shelves, glass door, gaskets, and inner chamber weekly (for cleaning see chap. 21.1, for
decontamination see chap. 21.2. You can clean the shelves in a laboratory dishwasher and, if needed,
individually autoclave them.
• Regularly use the hot air sterilization function (chap. 21.3) following cleaning. The shelves and the
emptied water pan can remain inside the chamber during this operation.
• Have replaced the CO
2
sterile filter (gas fine filter) once or twice a year.
What to do in case of contamination?
• Throw away / autoclave contaminated cultures.
• Carefully inspect cultures that seem to be uncontaminated.
• Clean the chamber as described. Wipe the inner chamber and the doors with a disinfectant and allow
drying. Autoclave the shelves. Empty the water pan and autoclave it.
• Perform a hot air sterilization.
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