nual 2 - Varispenser® 2x
E
N)
g
Manual
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Varispenser
®
2
Varispenser
®
2x
Operating Manual
0040 000 143-02/032022
Copyright© 2022 Eppendorf SE, Germany. All rights reserved, including graphics and
images. No part of this publication may be reproduced without the prior permission of the
copyright owner.
Brij™ is a trademark of LIFE TECHNOLOGIES, Netherlands.
Dismozon
®
is a registered trademark of Bode Chemie GmbH, Germany.
DNA AWAY™ is a trademark of Molecular Bio-Products Inc, USA.
Helipur
®
is a registered trademark of B. Braun Melsungen AG, Germany.
Hexaquart
®
is a registered trademark of B. Braun Melsungen AG, Germany.
Korsolex
®
is a registered trademark of Bode Chemie GmbH, Germany.
Meliseptol
®
is a registered trademark of B. Braun Melsungen AG, Germany.
RNase AWAY
®
is a registered trademark of Molecular Bio-Products Inc, USA.
Sterillium
®
is a registered trademark of Bode Chemie GmbH, Germany.
Tween
®
is a registered trademark of ICI Americas Inc, USA.
Eppendorf
®
and the Eppendorf Brand Design are registered trademarks of Eppendorf SE,
Germany.
Varispenser
®
is a registered trademark of Eppendorf SE, Germany.
Registered trademarks and protected trademarks are not marked in all cases with
®
or ™
in this manual.
Table of contents
Varispenser
®
2 - Varispenser
®
2x
English (EN)
3
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Other applicable documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.1 Physical properties of the liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.2 Unsuitable liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.3 Liquids with restricted use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Suitable acids and bases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.4 Suitable organic liquids. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.5 Suitable inorganic liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.6 Suitable saline solutions, buffers, wetting agents, oils, and
other solutions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.7 Suitable cleaning and decontamination agents . . . . . . . . . . . . . . . . . . . . . . 13
2.8 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.9 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.1.1 Varispenser 2 – 2 mL – 10 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.1.2 Varispenser 2 – 25 mL – 100 mL. . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.1.3 Varispenser 2x – 2 mL – 10 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.1.4 Varispenser 2x – 25 mL – 100 mL . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.2 Product overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.2.1 Varispenser 2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.2.2 Varispenser 2x . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.2.3 Telescopic aspirating tube. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.2.4 Recirculation tube – Varispenser 2x . . . . . . . . . . . . . . . . . . . . . . . 20
3.2.5 Thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
3.2.6 Ventilation screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
3.2.7 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
3.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
3.4 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4
Table of contents
Varispenser
®
2 - Varispenser
®
2x
English (EN)
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
4.1 Setting and inserting the telescopic aspirating tube . . . . . . . . . . . . . . . . . . 24
4.1.1 Adjusting the telescopic aspiration tube to the height
of the bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
4.1.2 Shortening the telescopic aspiration tube for smaller bottles. . . . 24
4.1.3 Inserting the telescopic aspirating tube . . . . . . . . . . . . . . . . . . . . 24
4.2 Installing the recirculation tube – Varispenser 2x . . . . . . . . . . . . . . . . . . . . 25
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.1 Screwing the dispenser onto the bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.2 Carrying the dispenser and the bottle. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.3 Screwing on the thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.3.1 Determining the diameter of the flask neck . . . . . . . . . . . . . . . . . 27
5.3.2 Screwing on the thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.4 Operating the volume selection slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.4.1 Unlocking the volume selection slider . . . . . . . . . . . . . . . . . . . . . 28
5.4.2 Setting the volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.4.3 Locking the volume selection slider . . . . . . . . . . . . . . . . . . . . . . . 29
5.5 Locking the piston. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.6 Dispensing liquid – Varispenser 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.6.1 Rinsing the dispenser prior to initial setup . . . . . . . . . . . . . . . . . . 30
5.6.2 Venting the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.6.3 Dispensing liquid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.6.4 Emptying the dispenser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.6.5 Rinsing the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.7 Dispensing liquid – Varispenser 2x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.7.1 Rinsing the dispenser prior to initial setup . . . . . . . . . . . . . . . . . . 33
5.7.2 Venting the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.7.3 Dispensing liquid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.7.4 Emptying the dispenser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.7.5 Rinsing the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.8 Cleaning the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.9 Rinsing the dispenser after using strong acids or bases . . . . . . . . . . . . . . . 38
6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.1 Dispenser and piston. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.2 Dispensing and liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.2 Autoclaving the dispenser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.3 Flushing a dispenser before long-term storage . . . . . . . . . . . . . . . . . . . . . . 43
Table of contents
Varispenser
®
2 - Varispenser
®
2x
English (EN)
5
7.4 Replacing valves or canula arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
7.4.1 Removing the filling valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
7.4.2 Mounting the filling valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
7.4.3 Disassembling the canula arm – Varispenser 2. . . . . . . . . . . . . . . 46
7.4.4 Disassembling the canula arm – Varispenser 2x. . . . . . . . . . . . . . 46
7.4.5 Disassembling the discharge valve . . . . . . . . . . . . . . . . . . . . . . . . 47
7.4.6 Mounting the discharge valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
7.4.7 Mounting the canula arm – Varispenser 2 . . . . . . . . . . . . . . . . . . 48
7.4.8 Mounting the canula arm – Varispenser 2x . . . . . . . . . . . . . . . . . 48
7.5 Adjusting the dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.5.1 Remove the adjustment cover. . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.5.2 Adjustment range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.5.3 Changing the adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.5.4 Checking the dispensing volume. . . . . . . . . . . . . . . . . . . . . . . . . . 50
8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1 Errors of measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1.1 Varispenser 2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1.2 Varispenser 2x . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
8.1.3 Test conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
8.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
9.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
9.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
9.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
10 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
10.1 Varispenser 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
10.2 Varispenser 2x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
10.3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
10.3.1 Telescopic aspirating tube. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
10.3.2 Flexible discharge tube with recirculation valve . . . . . . . . . . . . . . 57
10.3.3 Drying tube with sealing washer . . . . . . . . . . . . . . . . . . . . . . . . . . 58
10.3.4 Ventilation screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
10.3.5 Thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.4 Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
10.4.1 Canula arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
10.4.2 Sealing cap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
10.4.3 Discharge valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
10.4.4 Filling valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
10.4.5 Sealing washer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
10.4.6 Recirculation tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
10.4.7 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6
Operating instructions
Varispenser
®
2 - Varispenser
®
2x
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time.
Observe the instructions for use of the accessories where applicable.
This operating manual is part of the product. Please keep it in a place that is easily
accessible.
Enclose this operating manual when transferring the device to third parties.
The current version of the operating manual for all available languages can be found
on our webpage www.eppendorf.com/manuals
.
1.2 Danger symbols and danger levels
1.2.1 Danger symbols
The safety instructions in this manual have the following danger symbols and danger
levels:
1.2.2 Danger levels
1.3 Symbols used
Biohazard Toxic substances
Hazard point Material damage
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
Depiction Meaning
1.
2.
Actions in the specified order
Actions without a specified order
• List
Text Display or software texts
Additional information
Operating instructions
Varispenser
®
2 - Varispenser
®
2x
English (EN)
7
1.4 Other applicable documents
• Instructions for use for flexible discharge tube
• Instructions for use for drying tube
• SOP - Standard operating procedure for manual dispensing systems
8
Safety
Varispenser
®
2 - Varispenser
®
2x
English (EN)
2Safety
2.1 Intended use
The models of the Varispenser 2 series and the models of the Varispenser 2x series are
laboratory devices for dispensing aqueous solutions directly from a supply bottle. The
dispensers may only be used within the specified technical and physical limits.
Applications in or on the human body (in vivo applications) are not permitted.
The device has been designed for general laboratory applications and conforms to the
requirements of the relevant standards, e.g., DIN EN ISO 8655. The use of the device for
particular applications (e.g., for trace analysis, in the food industry, etc.) must be checked
meticulously by the user himself. Special permits for particular applications, e.g., for the
production or administration of food, pharmaceutics or cosmetics, have not been granted.
2.2 Application limits
2.2.1 Physical properties of the liquid
2.2.2 Unsuitable liquids
The dispenser is not suitable for the following solutions, substances, and liquids:
• Liquids with low ignition temperatures.
• Liquids which attack FEP, ETFE, PFA, PTFE, PVDF, PP, borosilicate glass or Al
2
O
3
ceramics.
• Solutions containing hydrofluoric acid.
• Suspensions, as solid particles can clog or damage the device (e.g. activated carbon).
• Liquids which form insoluble deposits and decomposing solutions (e.g. Biuret
reagent).
• Substances which react catalytically with platinum iridium (e.g. H
2
O
2
).
• Explosive liquids (e.g. carbon disulfide).
• Nitric acid > 60%
• Trifluoroacetic acid.
•Tetrahydrofuran.
Density
up to 2.2 g/cm
3
Vapor pressure up to 500 mbar *
Kinematic viscosity
up to 500 mm
2
/ s
Temperature 15 °C – 40 °C
* Above 300 mbar, aspirate the liquid slowly to avoid boiling of the liquid.
NOTICE! Material damage due to incorrect handling
Deposits that are hard to dissolve lead to irreparable damage to the piston, the
valves, and the discharge tube.
Only use approved liquids.
Safety
Varispenser
®
2 - Varispenser
®
2x
English (EN)
9
2.2.3 Liquids with restricted use
The dispenser is suitable for the following liquids to a limited extent:
• Dispense flammable liquids in glass tubes only and do not wipe the dispenser when it
is dry to avoid an electrostatic charge.
• Liquids which form soluble deposits can make the piston difficult to move.
• Use nitric acid (concentration 60 % maximum) with thread adapters made from ETFE
only.
2.3 Suitable acids and bases
Chemical substance Maximum concentration
Adipic acid unlimited
Aluminum hydroxide unlimited
Formic acid 98 % – 100 %
Ammonium hydroxide 20 %
Boric acid 10 %
Chloroacetic acid unlimited
Chromic acid
1
10 %
Chromic acid 50 %
Chromosulfuric acid unlimited
Acetic acid 50 %
Ethylenediaminetetraacetic acid unlimited
Potassium hydroxide
2
50 %
Lactic acid unlimited
Sodium hydroxide
2
30 %
Oxalic acid unlimited
Perchloric acid
1
10 %
Phosphoric acid 85 %
Nitric acid
1
60 %
Hydrochloric acid
2
35 %
Hydrochloric acid
1,
2
37 %
Salicylic acid unlimited
Sulfuric acid
1
98 %
Sulfuric acid 60 %
Tartaric acid unlimited
1
Use the thread adapter made from ETFE.
2
Use the drying tube.
10
Safety
Varispenser
®
2 - Varispenser
®
2x
English (EN)
2.4 Suitable organic liquids
Chemical substance Maximum concentration
Acetone unlimited
Acetonitrile unlimited
Acetylaldehyde unlimited
Benzene unlimited
Benzine unlimited
n-Butanol unlimited
n-Butyl acetate unlimited
Dibutyl phthalate
1
unlimited
Dichlorobenzene unlimited
Dichloroethane unlimited
Diethyl ether
1
unlimited
Diethylene glycol unlimited
Dimethylformamide
1
unlimited
1,4-Dioxane
1
unlimited
Acetic acid ethyl ester unlimited
Ethanol 100 %
Formaldehyde 40 %
Glycol unlimited
Heating oil (Diesel oil) unlimited
n-Hexane
1
unlimited
Isobutanol unlimited
Isopropanol unlimited
Methanol unlimited
Methylisobutylketone unlimited
Nitrobenzene
1
unlimited
Propanol unlimited
Octane
1
unlimited
Phenol (water saturated) unlimited
Pyridine
1
unlimited
Turpentine oil
1
unlimited
Toluol
1
unlimited
Safety
Varispenser
®
2 - Varispenser
®
2x
English (EN)
11
2.5 Suitable inorganic liquids
Trichloromethane (chloroform) unlimited
Triethylene glycol unlimited
Tripropylene glycol unlimited
Xylol unlimited
1
Use the thread adapter made from ETFE.
Chemical substance Maximum concentration
Aluminum chloride solution unlimited
Ammonium chloride solution unlimited
Ammonium fluoride unlimited
Barium chloride unlimited
Iodine potassium iodide solution unlimited
Calcium chloride unlimited
Potassium chloride unlimited
Potassium permanganate unlimited
Copper sulfate unlimited
Magnesium chloride unlimited
Mercury chloride unlimited
Silver nitrate unlimited
Zinc chloride 10 %
Zinc sulfate 10 %
Chemical substance Maximum concentration
12
Safety
Varispenser
®
2 - Varispenser
®
2x
English (EN)
2.6 Suitable saline solutions, buffers, wetting agents, oils, and other
solutions
Chemical substance Maximum concentration
Acrylonitrile
1
unlimited
Allyl alcohol unlimited
Amino acids unlimited
n-Amyl acetate unlimited
Amyl alcohol unlimited
Amyl chloride unlimited
Aniline unlimited
Benzaldehyde unlimited
Benzyl alcohol unlimited
Brij-35 or Brij unlimited
Dibutyl phthalate unlimited
Glycerol unlimited
Urea unlimited
m-Cresol unlimited
Methyl propyl ketone unlimited
Sodium acetate unlimited
Sodium dichromate unlimited
Sodium lauryl sulfate (SDS) unlimited
Propylene glycol unlimited
Propylene oxide unlimited
Salicylaldehyde unlimited
Silver acetate unlimited
TRIS HCl unlimited
Triton X-100 unlimited
Tween 20 unlimited
1
Use the thread adapter made from ETFE.
Safety
Varispenser
®
2 - Varispenser
®
2x
English (EN)
13
2.7 Suitable cleaning and decontamination agents
2.8 User profile
The device and accessories may only be operated by trained and skilled personnel.
Before using the device, read the operating manual and the instructions for use of the
accessories carefully and familiarize yourself with the device's mode of operation.
2.9 Warnings for intended use
Chemical substance Maximum concentration
Biocidal ZF unlimited
Cidex unlimited
Dismozon pur (peroxide-based) 4 %
DNA AWAY unlimited
DNA-Erase unlimited
Ethanol 70 %
Helipur (phenol-based) 6 %
Hexaquart S (QAV-based) 5 %
Hi-TOR Plus (DISCONTINUED) unlimited
Isopropanol 70 %
Korsolex basic (aldehyde-base) 5 %
Meliseptol (alcohol-based) unlimited
Sodium hypochlorite 4 %
RNase AWAY unlimited
RNase-ExitusPlus unlimited
Sterillium unlimited
WARNING! Damage to health due to infectious liquids and pathogenic
germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biosafety level of your laboratory, and the manufacturers'
Safety Data Sheets and application notes.
Wear your personal protective equipment.
Consult the "Laboratory Biosafety Manual" (source: World Health
Organization, Laboratory Biosafety Manual, as amended) for comprehensive
regulations on the handling of germs or biological material of risk group II or
higher.
14
Safety
Varispenser
®
2 - Varispenser
®
2x
English (EN)
WARNING! Damage to health due to toxic, radioactive or aggressive
chemicals.
Wear your personal protective equipment.
Observe the national regulations for handling these substances.
Observe the manufacturers' Safety Data Sheets and application notes.
CAUTION! Contamination due to contact with biological and chemical
reagents.
Contact with reagents may be harmful to eyes or skin.
Wear your personal protective equipment.
Make sure that no reagents are leaking from the device.
Ensure that the piston can move freely before starting work.
Do not point the opening of the discharge tube towards people.
Remove the sealing cap from the discharge tube before pushing down the
piston.
Only initiate liquid dispensing if you are not endangering other people.
Dispense liquid slowly and evenly to prevent splashing. Do not use force.
Only disassemble the device when it is clean.
CAUTION! Contamination with reagents when removing the sealing cap.
The sealing cap may contain biological and chemical reagents. Contact with
reagents may be harmful to eyes or skin.
Wear your personal protective equipment when removing the sealing cap.
CAUTION! Personal injury due to incorrect transportation of the device.
If the assembled device is not transported correctly, reagents will be released.
Contact with reagents may be harmful to eyes or skin.
To transport the assembled device, hold the head gear of the device with one
hand and support the bottom of the bottle with the other hand.
Do not hold the device by the cylinder sleeve.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by
Eppendorf may impair the safety, functioning and precision of the device.
Eppendorf cannot be held liable or accept any liability for damage resulting from
the use of accessories and spare parts other than those recommended or from
improper use.
Only use accessories and original spare parts recommended by Eppendorf.
Safety
Varispenser
®
2 - Varispenser
®
2x
English (EN)
15
NOTICE! Material damage due to improper use.
Only use the product for its intended purpose as described in the operating
manual.
Ensure adequate material resistance when using chemical substances.
If in doubt, contact the manufacturer of the product.
NOTICE! Damage to the device due to contamination inside the device.
If there are contaminations inside the dispenser, the dosing valve may be
blocked and the valve ball may be stuck. When the piston is pushed down, high
pressure develops in the dispenser. If the valve ball is not released, liquid is
pushed past the sealing lip and enters the inside of the housing.
If the piston is hard to move, clean the dispenser.
16
Product description
Varispenser
®
2 - Varispenser
®
2x
English (EN)
3 Product description
3.1 Delivery package
3.1.1 Varispenser 2 – 2 mL – 10 mL
3.1.2 Varispenser 2 – 25 mL – 100 mL
Quantity Description
1Varispenser 2
1 Operating manual
5 Thread adapter (25 mm, 28 mm, 32 mm, 38 mm, 40 mm)
1 Telescopic aspirating tube (125 mm – 240 mm)
1Universal wrench
1Certificate
Quantity Description
1Varispenser 2
1 Operating manual
3 Thread adapter (32 mm, 38 mm, 40 mm)
1 Telescopic aspirating tube (170 mm – 330 mm)
1Universal wrench
1Certificate
Product description
Varispenser
®
2 - Varispenser
®
2x
English (EN)
17
3.1.3 Varispenser 2x – 2 mL – 10 mL
3.1.4 Varispenser 2x – 25 mL – 100 mL
Quantity Description
1Varispenser 2x
1 Operating manual
5 Thread adapter (25 mm, 28 mm, 32 mm, 38 mm, 40 mm)
1 Telescopic aspirating tube (125 mm – 240 mm)
1Universal wrench
1Recirculation tube
1Certificate
Quantity Description
1Varispenser 2x
1 Operating manual
3 Thread adapter (32 mm, 38 mm, 40 mm)
1 Telescopic aspirating tube (170 mm – 330 mm)
1Universal wrench
1Recirculation tube
1Certificate
18
Product description
Varispenser
®
2 - Varispenser
®
2x
English (EN)
3.2 Product overview
3.2.1 Varispenser 2
Abb. 3-1:Varispenser 2
Fig. 3-1: Varispenser 2
1Piston bearing
2Volume scale
Maximum volume corresponds to the
nominal volume
3Piston pump
Housing, cylinder guard, cylinder und
piston
4 Ventilation screw
Connecting the optional accessories
(not included in the delivery package)
5 Threaded connection
6 Telescopic aspirating tube
7 Adjustment cover
8 Volume selection slider
9 Canula arm
10 Discharge tube
11 Sealing cap
1
2
3
4
5
6
8
9
10
11
7
Product description
Varispenser
®
2 - Varispenser
®
2x
English (EN)
19
3.2.2 Varispenser 2x
Abb. 3-2:Varispenser 2x
Fig. 3-2: Varispenser 2x
1Piston bearing
2Volume scale
Maximum volume corresponds to the
nominal volume
3Piston pump
Housing, cylinder guard, cylinder und
piston
4 Ventilation screw
Connecting the optional accessories
(not included in the delivery package)
5 Threaded connection
6 Telescopic aspirating tube
7 Adjustment cover
8 Volume selection slider
9Dosing valve
Including valve toggle
10 Canula arm
11 Discharge tube
12 Sealing cap
13 Recirculation tube
1
2
3
4
5
6
8
9
13
11
10
12
7
20
Product description
Varispenser
®
2 - Varispenser
®
2x
English (EN)
3.2.3 Telescopic aspirating tube
Abb. 3-3:Telescopic aspirating tube
Fig. 3-3: Telescopic aspirating tube
3.2.4 Recirculation tube – Varispenser 2x
Abb. 3-4:Recirculation tube
Fig. 3-4: Recirculation tube
1 Connection opening
Inner tube - connection side for filling
valve
2 Seal
3 Aspiration opening
Outer tube
1 Connection opening 2 Discharge opening
1
2
3
1
2
Product description
Varispenser
®
2 - Varispenser
®
2x
English (EN)
21
3.2.5 Thread adapter
Abb. 3-5:Thread adapter
Fig. 3-5: Thread adapter
3.2.6 Ventilation screw
The ventilation screw can be replaced by the optional accessories.
Optional accessories:
• Flexible discharge tube
•Drying tube
1 Reducing from 45 mm to 40 mm
2 Reducing from 45 mm to 38 mm
3 Reducing from 45 mm to 32 mm
4 Reducing from 32 mm to 28 mm
5 Reducing from 32 mm to 25 mm
1 2 3 4 5
22
Product description
Varispenser
®
2 - Varispenser
®
2x
English (EN)
3.2.7 Tools
Abb. 3-6:Universal wrench
Fig. 3-6: Universal wrench
3.3 Features
When handling aggressive liquids, the dispenser offers the greatest possible protection
for users and the laboratory environment. The user has to judge for himself whether the
Varispenser is suitable for the intended application. The user has to judge the chemical
compatibility of the reagents used himself.
The Varispenser is autoclavable.
Varispenser 2
The Varispenser 2 is a bottle top dispenser for dispensing liquids with milliliter accuracy.
• Dispensing - Dispense liquid from a bottle into a destination vessel.
• Adjustment option - Set the dispenser to liquids with a density other than water.
Varispenser 2x
The Varispenser 2x is a bottle top dispenser for dispensing liquids with milliliter accuracy
without wasting liquid.
• Dispensing - Dispense liquid from a bottle into a destination vessel.
• Recirculation - Recirculate liquid from the bottle back into the bottle, e.g., to remove
air bubbles from the cylinder.
• Adjustment option - Set the dispenser to liquids with a density other than water.
1Mounting wrench
Mounting side for valves
2 Adjustment wrench
1 2
Product description
Varispenser
®
2 - Varispenser
®
2x
English (EN)
23
3.4 Materials
When operated correctly, the dispensed liquid comes into contact with the following
chemically resistant materials: borosilicate glass, Al
2
O
3
ceramics, ETFE, FEP, PFA, PTFE,
platinum iridium, PP.
NOTICE! Aggressive substances may damage the Varispenser 2 and
accessories.
Check the chemical resistance before using organic solvents or aggressive
chemicals.
Assembly Material
Filling valve Perfluoroalkoxy (PFA), Al
2
O
3
ceramics, boroslilicate glass
Discharge tube Fluorinated ethylene propylene (FEP)
Discharge valve Perfluoroalkoxy (PFA), platinum-iridium (Pt-Ir), Al
2
O
3
ceramics, boroslilicate glass
Piston bearing Polypropylene (PP)
Sealing lip of the piston Perfluoroalkoxy (PFA)
Recirculation tube Fluorinated ethylene propylene (FEP)
Telescopic aspirating tube Fluorinated ethylene propylene (FEP),
polytetrafluorethylene (PTFE)
Valve ball (filling valve) Boroslilicate glass
Sealing cap Polypropylene (PP)
Volume selection switch Polypropylene (PP)
Cylinder Boroslilicate glass
24
Installation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
4 Installation
4.1 Setting and inserting the telescopic aspirating tube
4.1.1 Adjusting the telescopic aspiration tube to the height of the bottle
Prerequisites
• The bottle is larger than the collapsed telescopic aspirating tube.
4.1.2 Shortening the telescopic aspiration tube for smaller bottles
Prerequisites
• The bottle is smaller than the collapsed telescopic aspirating tube.
1. Extend the telescopic aspiration tube completely.
2. Shorten the inner tube at the connection side.
3. Shorten the outer tube opposite the aspiration openings.
4. Assemble the telescopic aspirating tube.
4.1.3 Inserting the telescopic aspirating tube
Prerequisites
• The telescopic aspiration tube is adjusted to the height of the bottle.
Use an empty bottle to adjust the telescopic aspiration tube.
1. Hold the telescopic aspiration tube next
to the bottle.
2. Extend the telescopic aspiration tube.
The telescopic aspiration tube is
supposed to reach from the bottle
opening to just above the base of the
bottle.
Installation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
25
4.2 Installing the recirculation tube – Varispenser 2x
Prerequisites
• The telescopic aspirating tube is inserted.
1. Push the telescopic aspirating tube
straight onto the filling valve until it
reaches the stop.
1. Insert the recirculation tube next to the
telescopic aspirating tube.
2. Rotate the opening of the recirculation
tube outward.
26
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
5Operation
5.1 Screwing the dispenser onto the bottle
Prerequisites
• Varispenser 2x – The recirculation tube is installed.
• The telescopic aspirating tube is installed.
• The length of the telescopic aspirating tube has been adjusted to the size of the bottle.
1. Put the dispenser straight onto the bottle
thread and tighten it.
2. Align the discharge tube to the label on
the bottle.
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
27
5.2 Carrying the dispenser and the bottle
5.3 Screwing on the thread adapter
The thread connection of the dispenser is designed for bottle threads of 45 mm. For other
bottle threads a thread adapter can be used. The diameter of the thread adapter is
imprinted on the adapter. If the bottle thread is smaller than 32 mm, a second adapter
must be used.
5.3.1 Determining the diameter of the flask neck
Prerequisites
• A suitable thread adapter is available.
Some thread adapters are included in the delivery package.Other diameters can be
ordered.
1. Measure the inner diameter of the bottle top or the outer diameter of the flask neck.
2. Choose the suitable thread adapter.
1. Grasp the dispenser by the thread
connection.
2. Hold the bottle at its base.
3. Carry the dispenser and the bottle
upright.
28
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
5.3.2 Screwing on the thread adapter
Prerequisites
• A suitable thread adapter is available.
1. Screw the thread adapter onto the flask neck.
The dispenser can be screwed onto the bottle.
5.4 Operating the volume selection slider
5.4.1 Unlocking the volume selection slider
5.4.2 Setting the volume
Prerequisites
• The volume selection slider is unlocked.
If you need a thread adapter with a higher resistance to chemicals, use a
PTFE/ETFE adapter.
1. Slide the volume selection slider to the
right.
The volume selection slider is unlocked.
The volume selection slider can be
moved.
The volume can be set.
1. Slide the volume selection slider to the
desired volume.
The volume has been set.
The volume selection slider can be
locked.
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
29
5.4.3 Locking the volume selection slider
5.5 Locking the piston
Prerequisites
• The volume selection slider is unlocked.
1. Slide the volume selector switch to the
left.
The volume selection slider is locked.
The volume selection slider cannot be
moved.
The liquid can be dispensed.
1. Slide the volume selection slider as far
upwards as it will go.
2. Slide the volume selection slider to the
left.
The volume selection slider is in the
basic position and it is locked.
No liquid can be dispensed.
30
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
5.6 Dispensing liquid – Varispenser 2
5.6.1 Rinsing the dispenser prior to initial setup
Prerequisites
• A bottle of demineralized water is available.
1. Screw the dispenser onto a bottle containing demineralized water.
2. Place a collection vessel below the discharge tube.
3. Remove the sealing cap carefully.
4. Pump several times to flush the dispenser.
5. Empty the dispenser.
6. Remove the dispenser and pump it through dry with air several times.
5.6.2 Venting the dispenser
Prerequisites
• The dispenser is clean.
• The dispenser has been screwed onto the storage bottle.
• The maximum volume is set.
• A collection vessel is available.
1. Remove the sealing cap carefully.
2. Hold a collection vessel below the discharge tube.
3. Pull up the piston by approx. 30 mm.
4. Dispense the liquid.
5. Repeat the procedure until the liquid in the cylinder is free from air bubbles.
6. Discard the liquid.
The dispenser has been vented.
The liquid can be dispensed accurately.
NOTICE! Damage to the device due to contamination inside the device.
If there are contaminations inside the dispenser, the dosing valve may be
blocked and the valve ball may be stuck. When the piston is pushed down, high
pressure develops in the dispenser. If the valve ball is not released, liquid is
pushed past the sealing lip and enters the inside of the housing.
If the piston is hard to move, clean the dispenser.
CAUTION! Contamination with reagents when removing the sealing cap.
The sealing cap may contain biological and chemical reagents. Contact with
reagents may be harmful to eyes or skin.
Wear your personal protective equipment when removing the sealing cap.
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
31
5.6.3 Dispensing liquid
Prerequisites
• The dispenser has been vented.
• A destination vessel is available.
5.6.4 Emptying the dispenser
Prerequisites
• The piston is in the lower position.
1. Set the required volume.
2. Remove the sealing cap carefully.
3. Hold a destination vessel under the
discharge tube.
4. Slowly and evenly pull the piston up until
the stop.
5. Slowly and evenly push the piston down.
The set amount of liquid is dispensed.
32
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
5.6.5 Rinsing the dispenser
Prerequisites
• The dispenser is empty.
• A bottle with a neutral cleaning solution is available.
• A bottle of demineralized water is available.
1. Screw the dispenser onto the bottle containing the neutral cleaning solution.
2. Place a collection vessel below the discharge tube.
3. Remove the sealing cap carefully.
4. Pump several times to flush the dispenser.
5. Empty the dispenser.
6. Screw the dispenser onto a bottle containing demineralized water.
7. Pump several times to flush the dispenser.
8. Empty the dispenser.
9. Remove the dispenser and pump it through dry with air several times.
1. Attach the sealing cap.
2. Place the dispenser and the bottle in a
collecting vessel.
3. Remove the dispenser.
4. Pull the dispenser out of the bottle until
the telescopic aspirating tube is no
longer immersed in the liquid.
5. Tap the telescopic aspirating tube
against the inside of the bottle.
The residual liquid flows out of the
telescopic aspirating tube.
The dispenser is empty.
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
33
5.7 Dispensing liquid – Varispenser 2x
5.7.1 Rinsing the dispenser prior to initial setup
Prerequisites
• The valve toggle is set to dispensing.
• A bottle of demineralized water is available.
1. Screw the dispenser onto a bottle containing demineralized water.
2. Place a collection vessel below the discharge tube.
3. Remove the sealing cap carefully.
4. Pump several times to flush the dispenser.
5. Set the valve toggle to recirculation.
6. Pump several times to flush the recirculation valve.
7. Empty the dispenser.
8. Remove the dispenser and pump it through dry with air several times.
NOTICE! Damage to the device due to contamination inside the device.
If there are contaminations inside the dispenser, the dosing valve may be
blocked and the valve ball may be stuck. When the piston is pushed down, high
pressure develops in the dispenser. If the valve ball is not released, liquid is
pushed past the sealing lip and enters the inside of the housing.
If the piston is hard to move, clean the dispenser.
CAUTION! Contamination with reagents when removing the sealing cap.
The sealing cap may contain biological and chemical reagents. Contact with
reagents may be harmful to eyes or skin.
Wear your personal protective equipment when removing the sealing cap.
34
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
5.7.2 Venting the dispenser
Prerequisites
• The dispenser is clean.
• The dispenser has been screwed onto the storage bottle.
• The maximum volume is set.
• A collection vessel is available.
5.7.3 Dispensing liquid
Prerequisites
• The dispenser has been vented.
• A destination vessel is available.
1. Remove the sealing cap carefully.
2. Set the valve toggle to recirculation.
3. Pull up the piston by approx. 30 mm.
4. Dispense liquid into the bottle.
5. Repeat the procedure until the liquid in
the cylinder is free from air bubbles.
6. Set the valve toggle to dispensing.
7. Fill the discharge tube with liquid.
8. Set the valve toggle to recirculation.
9. Dispense the remaining liquid into the
bottle.
10.Set the valve toggle to dispensing.
The dispenser has been vented.
Liquid can be dispensed.
1. Set the valve toggle to dispensing.
2. Remove the sealing cap carefully.
3. Set the required dispensing volume.
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
35
5.7.4 Emptying the dispenser
Prerequisites
• The piston is in the lower position.
• The valve toggle is set to dispensing.
4. Hold a collection vessel below the
discharge tube.
5. Slowly and evenly pull the piston up until
the stop.
6. Slowly and evenly push the piston down
until the stop.
The set amount of liquid is dispensed.
36
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
5.7.5 Rinsing the dispenser
Prerequisites
• The dispenser is empty.
• The valve toggle is set to dispensing.
• A bottle with a neutral cleaning solution is available.
• A bottle of demineralized water is available.
1. Screw the dispenser onto the bottle containing the neutral cleaning solution.
2. Place a collection vessel below the discharge tube.
3. Remove the sealing cap carefully.
4. Pump several times to flush the dispenser.
5. Set the valve toggle to recirculation.
6. Pump several times to flush the recirculation valve.
7. Empty the dispenser.
8. Screw the dispenser onto a bottle containing demineralized water.
9. Pump several times to flush the dispenser.
10.Pump several times to flush the recirculation valve.
11.Empty the dispenser.
12.Remove the dispenser and pump it through dry with air several times.
1. Attach the sealing cap.
2. Place the dispenser and the bottle in a
collecting vessel.
3. Remove the dispenser.
4. Pull the dispenser out of the bottle until
the telescopic aspirating tube is no
longer immersed in the liquid.
5. Tap the telescopic aspirating tube
against the inside of the bottle.
The residual liquid flows out of the
telescopic aspirating tube.
The dispenser is empty.
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
37
5.8 Cleaning the dispenser
The dispenser must be cleaned:
•If the piston is hard to move.
• Regularly for liquids which form soluble deposits.
• When the reagent is changed.
• Prior to autoclaving.
• Prior to storage.
• Prior to service and repair work.
• Prior to disassembly.
• Prior to valve replacement.
• Regularly when liquid accumulates in the sealing cap.
Prerequisites
• The dispenser is empty and flushed.
• A bottle of demineralized water is available.
• A bottle with a cleaning solution is available.
• A soft cleaning brush is available.
1. Pull off the telescopic aspirating tube and clean it with the cleaning brush.
2. Unscrew the piston mount and carefully pull out the piston.
3. Use the cleaning brush and water to clean the piston and cylinder.
4. Insert the piston into the cylinder.
5. Tighten the piston bearing.
6. Flush the dispenser with the cleaning solution.
7. Flush the dispenser with demineralized water.
8. Empty the dispenser.
CAUTION! Personal injury due to contact with reagents.
The feeding mechanism, valves, telescopic aspirating tube and discharge tube
are filled with reagents. Reagents enter the bottle with the cleaning solution.
Contact with reagents may be harmful to eyes or skin.
Wear your personal protective equipment.
Discard the cleaning solution after cleaning.
38
Operation
Varispenser
®
2 - Varispenser
®
2x
English (EN)
5.9 Rinsing the dispenser after using strong acids or bases
When strong acids or bases have been dispensed, the residual liquid in the dispenser
must first be neutralized.
• A bottle with neutralization liquid is available.
• A collection vessel is available.
• A bottle of demineralized water is available.
1. Screw the dispenser onto the bottle with neutralization liquid.
2. Remove the sealing cap.
3. Hold a collection vessel below the discharge tube.
4. Flush the dispenser several times.
5. Pump several times to flush the recirculation valve.
6. Remove the dispenser.
7. Screw the dispenser onto the bottle containing demineralized water.
8. Flush the dispenser several times.
9. Remove the dispenser.
10.Pump dry air through the dispenser several times.
Troubleshooting
Varispenser
®
2 - Varispenser
®
2x
English (EN)
39
6 Troubleshooting
6.1 Dispenser and piston
6.2 Dispensing and liquid
Problem Cause Solution
The piston is difficult
to move.
• The sealing cap is on the
discharge tube.
Remove the sealing cap
carefully.
• Crystals have formed. 1. Abort dispensing.
2. Clean the dispenser.
3. If the problem persists,
contact the authorized
service.
• The piston seal is damaged.
If the problem persists,
contact the authorized
service.
The canula arm cannot
be mounted.
• The discharge valve has not
been screwed far enough
into the valve block.
Use the universal wrench to
tighten the discharge valve.
Problem Cause Solution
The aspirated liquid
contains air bubbles.
• The dispenser was not
vented sufficiently.
Vent the dispenser.
• The telescopic aspirating
tube has not been mounted
correctly.
Slide the telescopic
aspirating tube firmly onto
the filling valve.
• The telescopic aspirating
tube is damaged.
Shorten the telescopic
aspirating tube or replace it.
• The filling valve is loose.
Use the universal wrench to
tighten the filling valve.
If the problem persists,
replace the filling valve with
a new valve.
• The filling valve is damaged.
If the problem persists,
replace the filling valve with
a new valve.
• The telescopic aspirating
tube is not immersed in the
liquid.
Lengthen the telescopic
aspirating tube until it is
immersed in the liquid .
• The bottle is empty.
Fill the bottle.
40
Troubleshooting
Varispenser
®
2 - Varispenser
®
2x
English (EN)
The dispenser does
not aspirate any liquid.
• The telescopic aspirating
tube has not been mounted
correctly.
Slide the telescopic
aspirating tube firmly onto
the filling valve.
• The filling valve is clogged. 1. Clean the dispenser.
2. If the problem persists,
replace the filling valve.
The dispensed volume
is too low.
• The telescopic aspirating
tube has not been mounted
correctly.
Slide the telescopic
aspirating tube firmly onto
the filling valve.
• The telescopic aspirating
tube is damaged.
Shorten the telescopic
aspirating tube or replace it.
• The dispenser is adjusted
incorrectly.
Adjust the dispenser.
If the problem persists, send
the dispenser to the
authorized service.
• The filling valve is loose.
Use the universal wrench to
tighten the filling valve.
If the problem persists,
replace the filling valve with
a new valve.
• The filling valve is damaged.
If the problem persists,
replace the filling valve with
a new valve.
No liquid can be
dispensed.
• The discharge valve is sticky.
Disassemble the discharge
valve.
Clean the discharge valve.
Release the jammed valve
ball.
If the problem persists,
replace the discharge valve
with a new valve.
Liquid escapes at the
threaded connection.
• The recirculation tube is
missing.
Insert the recirculation tube.
• A slightly volatile liquid was
dispensed without the
sealing washer.
Insert the sealing washer.
• Liquids that are sensitive to
moisture and/or CO
2
were
dispensed.
Insert the sealing washer.
Use the drying tube.
Problem Cause Solution
Troubleshooting
Varispenser
®
2 - Varispenser
®
2x
English (EN)
41
Liquid escapes at the
canula connection.
• The port of the discharge
valve is leaking.
Use the universal wrench to
tighten the discharge valve.
If the problem persists,
replace the discharge valve
with a new valve.
Liquid escapes
between the cylinder
and the housing cases.
• Cylinder or cylinder gasket
is leaking.
If the problem persists, send
the dispenser to the
authorized service.
Problem Cause Solution
42
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
7 Maintenance
7.1 Decontamination before shipment
If you are shipping the device to the authorized Technical Service for repairs or to your
authorized dealer for disposal please note the following:
7.2 Autoclaving the dispenser
Prerequisites
• The dispenser has been cleaned.
• Varispenser 2x – The valve toggle is set to dispensing.
• The dispensing piston is pressed down.
• Autoclave at 121 °C and 2 bar positive pressure.
1. Remove the sealing cap carefully.
2. Remove the telescopic aspirating tube.
3. Check the filling valve for proper seating.
4. Turn over the dispenser.
The filling valve faces upwards.
5. Slightly tap the valve block.
Any jammed valve balls are released.
The steam of the autoclave can pass through the valve without any obstruction.
6. Place the dispenser and the telescopic aspirating tube in the autoclave on a cloth.
7. Autoclave for 20 minutes.
8. Let the dispenser cool down for 2 hours after autoclaving.
9. Check all parts for distortion or leaks.
WARNING! Risk to health from contaminated device.
1. Observe the information contained in the decontamination certificate. It is
available as a PDF document on our webpage
(https://www.eppendorf.com/decontamination
).
2. Decontaminate all parts to be shipped.
3. Include the fully completed decontamination certificate in the shipment.
NOTICE! Material damage from hot metal surfaces.
The plastic parts of the dispenser can melt if they get into contact with hot metal
surfaces.
Place the dispenser on a cloth during autoclaving.
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
43
7.3 Flushing a dispenser before long-term storage
Prerequisites
• A bottle of demineralized water is available.
• A bottle with a mixture of glycerol (1 %) in ethanol (reagent-grade, not denatured) is
available.
• A collection vessel is available.
1. Screw the dispenser onto the bottle containing demineralized water.
2. Set the volume selection slider to maximum volume.
3. Remove the sealing cap carefully.
4. Flush the dispenser several times.
5. Remove the dispenser.
6. Screw the dispenser onto the bottle containing the glycerol-ethanol mixture.
7. Flush the dispenser several times.
8. Remove the dispenser.
9. Tap the telescopic aspirating tube against the inside of the bottle.
The residual liquid flows out of the telescopic aspirating tube.
The dispenser is empty.
44
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
7.4 Replacing valves or canula arm
Abb. 7-1:Valve block with valves
Fig. 7-1: Valve block with valves
CAUTION! Personal injury due to contact with reagents.
The feeding mechanism, valves, telescopic aspirating tube and discharge tube
are filled with reagents.
Contact with reagents may be harmful to eyes or skin.
Only disassemble the device if it is clean and has been decontaminated.
1Valve block
With filling valve und discharge valve
2Filling valve
3Discharge valve
4Housing
5 Canula arm
Varispenser 2
6Valve toggle
7Housing
8Dosing valve
9 Canula arm
Varispenser 2x
2
1
4
7 8
3
5
9
6
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
45
7.4.1 Removing the filling valve
Prerequisites
• The dispenser is clean.
• The telescopic aspirating tube has been removed.
• Varispenser 2x – The recirculation tube has been removed.
• The universal wrench is available.
7.4.2 Mounting the filling valve
Prerequisites
• The defective filling valve has been removed.
• A new filling valve is available.
• The universal wrench is available.
1. Attach the universal wrench to the top of
the filling valve.
2. Unscrew the filling valve in a
counterclockwise direction.
1. Screw a new filling valve into the valve
block by hand.
2. Attach the universal wrench.
3. Tighten the filling valve in a clockwise
direction.
46
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
7.4.3 Disassembling the canula arm – Varispenser 2
7.4.4 Disassembling the canula arm – Varispenser 2x
1. Push up the housing of the canula arm
on the valve block.
2. Remove the canula arm in a forward
motion.
1. Pull off the valve toggle.
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
47
7.4.5 Disassembling the discharge valve
Prerequisites
• The canula arm has bee disassembled.
2. Push up the housing of the canula arm
on the valve block.
3. Remove the canula arm in a forward
motion.
1. Attach the universal wrench to the top of
the discharge valve.
2. Unscrew the discharge valve in a
counterclockwise direction.
48
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
7.4.6 Mounting the discharge valve
Prerequisites
• The defective discharge valve has been removed.
• A new discharge valve is available.
• The universal wrench is available.
7.4.7 Mounting the canula arm – Varispenser 2
7.4.8 Mounting the canula arm – Varispenser 2x
Prerequisites
• The valve toggle has been taken off.
1. Screw a new discharge valve into the
valve block by hand.
2. Attach the universal wrench.
3. Tighten the discharge valve in a
clockwise direction.
1. Insert the dosing valve into the discharge
valve on the valve block.
2. Slide the housing over the lug on the
valve block.
3. Push the housing down.
The housing engages in the lower lugs.
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
49
7.5 Adjusting the dispenser
The dispenser has been adjusted to the physical properties of demineralized water at the
factory.
The dispenser can be readjusted:
• If the physical properties of the liquid are different to those of water.
• If the error of measurement of the dispensed volume is outside of the tolerance range.
7.5.1 Remove the adjustment cover.
Prerequisites
• The universal wrench is available.
1. Push the dosing valve slightly downward
in the housing.
2. Slide the dosing valve into the valve
block.
The housing of the canula arm must be
above the lug.
3. Push the housing down.
The housing engages.
4. Attach the valve toggle to the top of the
dosing valve.
The gravimetric test and the conversion of the measured values for the volume
are described in the document “Standard operating procedure for manual
dispensing systems". The document is available on the webpage
www.eppendorf.com/manuals
www.eppendorf-support.com.
Perform another gravimetric check after adjusting the dispenser.
1. Insert the adjustment wrench of the
universal wrench into the adjustment
cover.
2. Turn the adjustment wrench and break
off the adjustment cover.
The adjustment cover can be disposed
of.
50
Maintenance
Varispenser
®
2 - Varispenser
®
2x
English (EN)
7.5.2 Adjustment range
7.5.3 Changing the adjustment
Prerequisites
• The gravimetrically determined measured values are available.
7.5.4 Checking the dispensing volume
Prerequisites
• The document "Standard operating procedure for manual dispensing systems" is
available.
Perform a gravimetric check of the dispensed volume.
Nominal volume Maximum ± per revolution
2 mL 12 μL ~ 16 μL
5 mL 30 μL ~ 40μL
10 mL 60 μL ~ 80 μL
25 mL 150 μL ~ 130 μL
50 mL 300 μL ~ 265 μL
100 mL 600 μL ~ 400 μL
Insert the adjustment wrench into the
adjustment opening.
Turning in the plus direction increases
the dispensing volume.
Turning in the minus direction decreases
the dispensing volume.
+-
Technical data
Varispenser
®
2 - Varispenser
®
2x
English (EN)
51
8 Technical data
8.1 Errors of measurement
8.1.1 Varispenser 2
Model Testing volume Error of measurement
Systematic Random
± % ± µL ± % ± µL
0.2 mL – 2 mL 0.2 mL 5 10 1 2
1 mL 1 10 0.2 2
2 mL 0.5 10 0.1 2
0.5 mL – 5 mL 0.5 mL 5 25 1 5
2.5 mL 1 25 0.2 5
5 mL 0.5 25 0.1 5
1 mL – 10 mL 1 mL 5 50 1 10
5 mL 1 50 0.2 10
10 mL 0.5 50 0.1 10
2.5 mL – 25 mL 2.5 mL 5 125 1 25
12.5 mL 1 125 0.2 25
25 mL 0.5 125 0.1 25
5 mL – 50mL 5 mL 5 250 1 50
25 mL 1 250 0.2 50
50 mL 0.5 250 0.1 50
10 mL – 100 mL 10 mL 5 500 1 100
50 mL 1 500 0.2 100
100 mL 0.5 500 0.1 100
52
Technical data
Varispenser
®
2 - Varispenser
®
2x
English (EN)
8.1.2 Varispenser 2x
8.1.3 Test conditions
Test conditions and test analysis in accordance with ISO 8655-6.
8.2 Ambient conditions
Model Testing volume Error of measurement
Systematic Random
± % ± µL ± % ± µL
0.2 mL – 2 mL 0.2 mL 5 10 1 2
1 mL 1 10 0.2 2
2 mL 0.5 10 0.1 2
0.5 mL – 5 mL 0.5 mL 5 25 1 5
2.5 mL 1 25 0.2 5
5 mL 0.5 25 0.1 5
1 mL – 10 mL 1 mL 5 50 1 10
5 mL 1 50 0.2 10
10 mL 0.5 50 0.1 10
2,5 mL – 25 mL 2.5 mL 5 125 1 25
12.5 mL 1 125 0.2 25
25 mL 0.5 125 0.1 25
5 mL – 50 mL 5 mL 5 250 1 50
25 mL 1 250 0.2 50
50 mL 0.5 250 0.1 50
10 mL – 100 mL 10 mL 5 500 1 100
50 mL 1 500 0.2 100
100 mL 0.5 500 0.1 100
Ambience Only for use indoors.
Ambient temperature 15 °C – 40 °C
Relative humidity 10 % – 90 %, non-condensing.
Atmospheric pressure 700 hPa – 1060 hPa
Transport, storage and disposal
Varispenser
®
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®
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53
9 Transport, storage and disposal
9.1 Transport
Tab. 9-1: Transport conditions
9.2 Storage
Tab. 9-2: Storage conditions
9.3 Disposal
Observe the relevant legal regulations for disposing of the product.
NOTICE! Damage due to incorrect packing.
Eppendorf SE is not liable for any damage caused by improper packing.
Only store and transport the device in its original packing.
Air temperature Relative humidity Atmospheric pressure
General transport -20 °C – 50 °C 10 % – 90 %,
non-condensing.
300 hPa – 1060 hPa
Air freight -20 °C – 50 °C 10 % – 90 %,
non-condensing.
300 hPa – 1060 hPa
Air temperature Relative humidity Atmospheric pressure
Storage -20 °C – 50 °C 10 % – 90 %,
non-condensing.
300 hPa – 1060 hPa
54
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
10 Ordering Information
10.1 Varispenser 2
10.2 Varispenser 2x
Order no.
(International)
Order no.
(North America)
Description
Varispenser 2
Bottle top dispenser for bottle thread 45 mm,
telescopic aspirating tube, universal wrench,
5 adapters (25 mm, 28 mm, 32 mm, 38 mm,
40 mm)
4966 000.010 4966000010 0.2 mL – 2 mL
4966 000.029 4966000029 0.5 mL – 5 mL
4966 000.037 4966000037 1 mL – 10 mL
Varispenser 2
Bottle top dispenser for bottle thread 45 mm,
telescopic aspirating tube, universal wrench, 3
adapters (32 mm, 38 mm, 40 mm)
4966 000.045 4966000045 2.5 mL – 25 mL
4966 000.053 4966000053 5 mL – 50 mL
4966 000.061 4966000061 10 mL – 100 mL
Order no.
(International)
Order no.
(North America)
Description
Varispenser 2x
Bottle top dispenser with return valve,
and valve switch for bottle thread
45 mm, telescopic aspirating tube,
universal wrench, 5 adapters (25 mm,
28 mm, 32 mm, 38 mm, 40 mm)
4967 000.014 4967000014 0.2 mL – 2 mL
4967 000.022 4967000022 0.5 mL – 5 mL
4967 000.030 4967000030 1 mL – 10 mL
Varispenser 2x
Bottle top dispenser with return valve,
and valve switch for bottle thread
45 mm, telescopic aspirating tube,
universal wrench, 3 adapters (32 mm,
38 mm, 40 mm)
4967 000.049 4967000049 2.5 mL – 25 mL
4967 000.057 4967000057 5 mL – 50 mL
4967 000.065 4967000065 10 mL – 100 mL
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
55
10.3 Accessories
1 Ventilation screw
2Filling valve
3 Telescopic aspirating tube
4Discharge valve
5 Recirculation tube
Varispenser 2x
6 Canula arm
With discharge tube for Varispenser 2
7 Canula arm
With discharge tube and valve toggle for
Varispenser 2x
8 Sealing cap
2
1
3
6
7
8
4
5
56
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
10.3.1 Telescopic aspirating tube
Order no.
(International)
Order no.
(North America)
Description
Telescopic aspirating tube
70 mm – 140 mm
4966 503.004 4966503004 for 2 mL, 5 mL, 10 mL
Telescopic aspirating tube
125 mm – 240 mm
4966 504.000 4966504000 for 2 mL, 5 mL, 10 mL
Telescopic aspirating tube
195 mm – 350 mm
4966 505.007 4966505007 for 2 mL, 5 mL, 10 mL
Telescopic aspirating tube
250 mm – 480 mm
4966 506.003 4966506003 for 2 mL, 5 mL, 10 mL
4966 508.006 4966508006 for 25 mL, 50 mL, 100 mL
Telescopic aspirating tube
170 mm – 330 mm
4966 507.000 4966507000 for 25 mL, 50 mL, 100 mL
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
57
10.3.2 Flexible discharge tube with recirculation valve
Order no.
(International)
Order no.
(North America)
Description
Discharge tube
spiraled
4966 501.001 4966501001 for 2 mL, 5 mL, 10 mL
4966 502.008 4966502008 for 25 mL, 50 mL, 100 mL
58
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
10.3.3 Drying tube with sealing washer
10.3.4 Ventilation screw
Order no.
(International)
Order no.
(North America)
Description
Drying tube
4966 509.002 4966509002 without filling, including sealing washer (PTFE)
Order no.
(International)
Order no.
(North America)
Description
4966 601.006 4966601006 Ventilation screw
PP
4966 511.007 4966511007 Ventilation screw
for microfilters with Luer connector, PP, with
PTFE sealing washer
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
59
10.3.5 Thread adapter
• GL – round thread DIN 138 for glass threads. Number indicates the maximum outer
diameter of the male thread.
• S – buttress thread. Thread type for glass bottles seals which is to prevent them
becoming loose unintentionally due to vibrations.
Order no.
(International)
Order no.
(North America)
Description
Bottle thread adapter
PP
4960 800.040 4960800040 from GL 32 to GL 25,
4960 800.139 4960800139 from GL 32 to GL 27
4960 800.058 4960800058 from GL 32 to GL/S 28
4960 800.120 4960800120 from GL 45 to GL 32
4960 800.155 4960800155 from GL 45 to GL 38
4960 800.147 4960800147 from GL 45 to S 40
Bottle thread adapter
ETFE
4966 614.000 4966614000 from GL 32 to GL 25
4960 835.005 4960835005 from GL 32 to GL/S 28
4966 615.007 4966615007 from GL 45 to GL 32
4960 839.000 4960839000 from GL 45 to GL 38
Bottle thread adapter
PTFE
4960 834.009 4960834009 from GL 45 to S 40
Thread adapter
for 5 L-jerrycan, ETFE
4960 832.006 4960832006 from 45 mm to 17/8" thread
45-40 45-38 45-32
32-28
32-25
60
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
10.4 Spare parts
10.4.1 Canula arm
1Varispenser 2x
Dosing valve, valve toggle, discharge
tube und sealing cap
2Varispenser 2
Discharge tube und sealing cap
Order no.
(International)
Order no.
(North America)
Description
Discharge tube
Varispenser 2
4966 608.000 4966608000 for 2 mL, 5 mL, 10 mL
4966 609.007 4966609007 for 25 mL, 50 mL, 100 mL
Discharge tube
Varispenser 2x
4967 601.000 4967601000 for 2 mL, 5 mL, 10 mL
4967 602.006 4967602006 for 25 mL, 50 mL, 100 mL
1 2
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
61
10.4.2 Sealing cap
10.4.3 Discharge valve
Order no.
(International)
Order no.
(North America)
Description
Sealing cap
PP, blue
4966 611.001 4966611001 for 2 mL, 5 mL, 10 mL
4966 612.008 4966612008 for 25 mL, 50 mL, 100 mL
Order no.
(International)
Order no.
(North America)
Description
Discharge valve
4966 604.005 4966604005 for 2 mL
4966 605.001 4966605001 for 5 mL, 10 mL
4966 606.008 4966606008 for 25 mL, 50 mL, 100 mL
62
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
10.4.4 Filling valve
10.4.5 Sealing washer
Order no.
(International)
Order no.
(North America)
Description
Filling valve
with valve ball
4966 602.002 4966602002 for 2 mL, 5 mL, 10 mL
4966 603.009 4966603009 for 25 mL, 50 mL, 100 mL
Order no.
(International)
Order no.
(North America)
Description
4966 613.004 4966613004 Sealing washer
for valve block, PTFE
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
63
10.4.6 Recirculation tube
10.4.7 Tools
Order no.
(International)
Order no. (North
America)
Description
4966 610.005 4966610005 Recirculation tube
FEP
Order no.
(International)
Order no.
(North America)
Description
4966 607.004 4966607004 Universal wrench
64
Ordering Information
Varispenser
®
2 - Varispenser
®
2x
English (EN)
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