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c. Attach a Cover Slide (N) over the specimen and place the slide onto the microscope stage,
align and focus.
d. If you wish to save the slide, place Gum Media on a clean dry slide before placing the specimen
on the slide, and into the media. Place a Cover Slide over the media and allow to dry.
7. Staining Smears: Staining a smear can help observing specimens that may be hard to see. The first
step in staining a smear is creating the stain.
a. Your kit comes with Eosin Dye in powder form. Locate the plastic container with the Eosin Dye.
b. Carefully remove the cap of the container. Use your plastic Dropper to add water to the container
until it is full.
c. Gently stir the mixture until the powder is infused with the water. The Dye is now ready to use.
d. Create a Smear as described previously and do not place any water or a cover slip on the specimen.
e. Set the slide aside to dry.
f. Once dry, use the Dropper to place one drop of the liquid Dye on the slide.
g. Tilt the Slide from side to side to spread the stain over the specimen.
h. Remove the excess fluid to the “Waste” cup.
i. Let the slide dry for a several minutes.
j. Once dry, you are ready to observe.
8. Life Under Glass:
a. Fill the wide mouthed jar with fresh water.
b. Drop a handful of grass and a pinch or two of dirt into the water.
c. Put the lid on the jar and place it in an area that will get moderate sunlight.
d. Let it stand for 3-4 days.
e. After 5 days, it is time to examine the water.
f. Make a specimen slide by using a clean slide and petroleum jelly.
g. Use a toothpick to make a ring with the petroleum jelly on the slide surface.
h. The ring should be smaller than a cover slip and half as thick as the slide thickness.
i. Put a drop of the jar water inside the ring.
j. Using the lowest power on the microscope (shortest objective), observe the specimen.
k. Take note of any microbial movement in the water.
l. Have fun and write down your observations.
CARING FOR YOUR MICROSCOPE: Your microscope is a precision optical instrument and when treated
with care, should provide years of use. Here are a few tips to keep your microscope in top shape:
• Always carry the microscope with two hands.
• Always remove slides from the stage before putting the microscope away.
• Cover the microscope or place it in the plastic carrying case, when not in use.
• Use lens cleaning tissue ONLY when cleaning the lenses.
• Never allow the objective lenses to touch the slide or the stage.
• Remove the batteries before storing the microscope for extended periods of time (30 days
or longer).
7
MICROSCOPEKIT
28
PIECE
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